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Plant Growth Regulation

, Volume 16, Issue 1, pp 99–101 | Cite as

Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis

  • Yongqin Chen
  • Chitrapan Piluek
Short Communication

Abstract

Adventitious bud formation from the vegetative buds of the flower stalks of Phalaenopsis occurred on Vacin and Went medium with 15% coconut water and 5 to 40 μM thidiazuron (TDZ) or 40 μM N6-benzylaminopurine. The highest efficiency of induction was achieved with 5 or 10 μM TDZ. Adventitious buds developed into shoots on VWC medium. TDZ was more effective than BAP in stimulating the axillary buds of intact shoots to develop. Regenerated shoots rooted after about two months of culture on VWC medium with 1% sucrose. Shoot tips excised from the regenerated shoots initiated protocorm-like bodies after two months of culture on VWC medium.

Key words

adventitious buds aseptic culture benzylaminopurine flower stalks Phalaenopsis protocorm-like bodies thidiazuron 

Abbreviations

VWC medium

Vacin and Went medium with 15% (by volume) coconut water

TDZ

thidiazuron

BAP

N6-benzylaminopurine

Plbs

protocorm-like bodies

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Yongqin Chen
    • 1
  • Chitrapan Piluek
    • 1
  1. 1.Department of HorticultureKasetsart UniversityBangkokThailand

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