Advertisement

Euphytica

, Volume 47, Issue 1, pp 33–38 | Cite as

Variation amongst protoplast-derived moth bean Vigna aconitifolia plants

  • I. D. Arya
  • Sarita Arya
  • D. V. Rao
  • N. S. Shekhawat
Article

Summary

Protoplasts were isolated from leaves of a single plant of Vigna aconitifolia, a drought resistant grain legume. The protoplasts regenerated and formed colony and calli from which 50 entire plants were regenerated and transferred to field conditions. Only 7 plants survived upto maturity and they flowered and produced pods with seed. The protoplast derived plants showed variation in important characters. Two groups of characters (one with 7 sets and another with 6 sets) were studied in the protoclones. In first group protoclones showed variations in seed germination, maturity age, pod length, pod and seed colour, number of abortive seed per pod and response to field rots, however, not much difference was recorded in pollen stainability and meiotic behaviour in these protoclones. In second group analysis of variants showed significance difference for plant height, rachis-length, length and breadth of mature odd leaflets, seed per pod and weight of seed. The results indicate that protoplast can be source of variation in this crop. However, detailed biochemical and genetical analysis of protoclones are required.

Key words

Vigna aconitifolia moth bean protoplast culture protoclones in vitro variations 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barden, K.A., S.S.Smith & H.H.Murakishi, 1986. Regeneration and screening of tomato somaclones for resistance to tobacco mosaic virus. Plant Science 45: 209–214.Google Scholar
  2. Bhargava, S. & N.Chandra, 1983. In vitro differentiation in callus cultures of moth bean, Vigna aconitifolia (Jacq.) Maréchal. Plant Cell Reports 2: 47–50.Google Scholar
  3. Dale, P.J., 1983. Protoplast Culture and Plant regeneration of cereals and other recalcitrant crops. Experimentia 46(S): 31–41.Google Scholar
  4. Eapen, S. & R.Gill, 1986. Regeneration of Plants from Cultured root explants of mothbean Vigna aconitifolia. Theor. Appl. Genet. 72: 384–387.Google Scholar
  5. Evans, D.A., W.R.Sharp & H.P.Medina-Filho, 1984. Somaclonal and gametoclonal variation. Am. J. Bot. 71: 759–774.Google Scholar
  6. Godbole, D.A., M.N.Kunachgi, U.A.Potdar, K.V.Krishnamurthy & A.F.Mascarenhas, 1984. Studies on a drought resistant: The Moth bean Vigna aconitifolia (Jacq.) Maréchal. II. Morphogenetic studies. Plant Cell Reports 3: 75–78.Google Scholar
  7. Hughes, K.W., 1986. Culture derived crossing over barriers. Am. J. Bot. 73: 123–129.Google Scholar
  8. Karp, A., R.S.Nelson, E.Thomas & S.W.J.Bright, 1982. Chromosome variation in protoplast-derived potato plants. Theor. Appl. Genet. 63: 265–272.Google Scholar
  9. Kaur-Sawhney, R., N.S.Shekhawat & A.W.Galston, 1985. Polyamine levels as related to growth, differentiation, and senescene in protoplast derived cultures of Vigna aconitifolia and Avena sativa. Plant Growth Regulation 3: 329–337.Google Scholar
  10. Krishnamurthy, K.V., D.A.Godbole & A.F.Mascarenhas, 1984. Studies on drought resistant legume: the moth bean, Vigna aconitifolia (Jacq.) Maréchal. I. Protoplast Culture and Organogenesis. Plant Cell Reports 3: 30–32.Google Scholar
  11. Landsmann, J. & H.Uhrig, 1985. Somaclonal variation in Solanum tuberosum detected at the molecular level. Theor. App. Genet. 71: 500–505.Google Scholar
  12. Larkin, P.J. & W.R.Scowcroft, 1981. Somaclonal variation a novel source of variability from cell cultures for Plant improvement. Theor. Appl. Genet. 60: 197–214.Google Scholar
  13. Larkin, P.J., R.Brettell, S.Ryan & W.Scowcroft, 1983. Protoplasts and variation from culture. Experimentia 46(S): 51–56.Google Scholar
  14. Larkin, P.J., S.A.Ryan, R.Brettell & W.R.Scowcroft, 1984. Heritable somaclonal variation in wheat. Theor. Appl. Genet. 67: 443–455.Google Scholar
  15. Latunde-Dada, A.O. & J.A.Lucas, 1983. Somaclonal Variation and reaction to Verticillium wilt in Medicago sativa L. Plants regenerated from protoplasts. Plant Science Letters 32: 205–211.Google Scholar
  16. Lorz, H. & W.R.Scowcroft, 1983. Variability among plants and their progeny regenerated from protoplasts of Su/su heterozygotes of Nicotiana tabacum. Theor. Appl. Genet. 66: 67–75.Google Scholar
  17. Panse, V.G. & P.V.Sukhatme, 1967. Statistical methods for agricultural workers. ICAR, New Delhi.Google Scholar
  18. Secor, G.A. & J.F.Shepard, 1981. Variability of protoplast derived potato clones. Crop Science 21: 102–105.Google Scholar
  19. Sharma, A.K. & A. Sharma, 1972. Chromosome Techniques. Theory and Practice. Butterworths University. Park Press pp. 109–110.Google Scholar
  20. Shekhawat, N.S. & A.W.Galston, 1983. Isolation, Culture and Regeneration of moth bean Vigna aconitifolia leaf protoplasts. Plant Science Letters 32: 43–51.Google Scholar
  21. Shekhawat, N.S., P.N.Gordon & A.W.Galston, 1984. Highly regenerative tissue cultures from axillary tiller buds of sexually mature oat plants (Avena sativa L.) in vitro. 20: 707–711.Google Scholar
  22. Sibi, M., 1971. Creation de variabilité par culture de tissue in vitro chez Lactuca sativa. DEA Amél. Plantes Université Paris Sud ORSAY.Google Scholar
  23. Sibi, M., 1976. La notion de programme génétique chez les végétaux supérieurs - Ann. Amel. Plantes. 26: 523–547.Google Scholar
  24. Sree Ramulu, K., P.Dijkhuis & S.Roest, 1983. Phenotypic variation and ploidy level of plants regenerated from Protoplasts of tetraploid potato (Solanum tuberosum L. cv. Bintje). Theor. Appl. Genet. 65: 329–338.Google Scholar
  25. Sree Ramulu, K., P.Dijkhuis, S.Roest, G.S.Bokelmann & B.D.Root, 1984. Early occurrence of genetic instability in protoplast cultures of potato. Plant Science Letters, 36: 79–86.Google Scholar
  26. Thomas, E., S.W.J.Bright, J.Frnklin, V.A.Lancaster & B.J.Miflin, 1982. Variation amongst protoplast derived potato plants (Solanum tuberosum) cv. Maris Bard. Theor. Appl. Genet. 62: 65–68.Google Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • I. D. Arya
    • 1
  • Sarita Arya
    • 1
  • D. V. Rao
    • 1
  • N. S. Shekhawat
    • 1
  1. 1.Department of BotanyUniversity of JodhpurJodhpurIndia

Personalised recommendations