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Plant Cell, Tissue and Organ Culture

, Volume 5, Issue 2, pp 129–138 | Cite as

Plant regeneration of Solanum lycopersicoides Dun. from stem explants, callus and suspension cultures

  • L. W. Handley
  • K. C. Sink
Article

Abstract

Protocols were established for achieving plant regeneration from stem internode, callus, and cell suspension cultures of Solanum lycopersicoides Dun. Two accessions of S. lycopersicoides exhibited different responses as to callus formation on various media, requirement of gibberellic acid for shoot regeneration, and ability to grow in suspension culture. The optimum medium for initiation and maintenance of cell suspension cultures was Murashige and Skoog [9] medium with 15 mg l NAA. For shoot regeneration, of three cytokinins tested, zeatin was found most effective relative to number, rapidity of response and overall quality of shoots. Shoot regeneration from stem explants, callus and suspension cultures was optimum on MS + 3.0 mg l−1 zeatin + 0.1 mg l−1 gibberellic acid.

Key words

Solanum lycopersicoides tomato callus suspension regeneration embryo-genesis gibberellic acid 

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Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1985

Authors and Affiliations

  • L. W. Handley
    • 1
  • K. C. Sink
    • 1
  1. 1.Department of HorticultureMichigan State UniversityEast LansingUSA

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