Plant Cell, Tissue and Organ Culture

, Volume 5, Issue 2, pp 129–138 | Cite as

Plant regeneration of Solanum lycopersicoides Dun. from stem explants, callus and suspension cultures

  • L. W. Handley
  • K. C. Sink


Protocols were established for achieving plant regeneration from stem internode, callus, and cell suspension cultures of Solanum lycopersicoides Dun. Two accessions of S. lycopersicoides exhibited different responses as to callus formation on various media, requirement of gibberellic acid for shoot regeneration, and ability to grow in suspension culture. The optimum medium for initiation and maintenance of cell suspension cultures was Murashige and Skoog [9] medium with 15 mg l NAA. For shoot regeneration, of three cytokinins tested, zeatin was found most effective relative to number, rapidity of response and overall quality of shoots. Shoot regeneration from stem explants, callus and suspension cultures was optimum on MS + 3.0 mg l−1 zeatin + 0.1 mg l−1 gibberellic acid.

Key words

Solanum lycopersicoides tomato callus suspension regeneration embryo-genesis gibberellic acid 


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  1. 1.
    Breidenbach RW, Waring AJ (1977) Response to chilling of tomato seedlings and cells in suspension cultures. Plant Physiol 60:190–192Google Scholar
  2. 2.
    Gleddie S, Keller W, Setterfield G (1983) Somatic embryogenesis and plant regeneration from leaf explants and cell suspensions of Solanum melongena (eggplant). Can J Bot 61:656–666Google Scholar
  3. 3.
    Halperin W, Wetherell F (1964) Adventive embryony in tissue cultures of the wild carrot, Daucus carota. Am J Bot 51:274–283Google Scholar
  4. 4.
    Herner RC (1982) Chilling injury in tomato. Report to a symposium: Plant responses to environmental stress. Annual meeting AAAS. WashingtonGoogle Scholar
  5. 5.
    Herner RC, Kamps T (1983) Chilling injury tolerance of wild tomato species. Proc. 4th Tomato Quality Workshop. March 7–10. Veg Crops Res RPT VEC-83-1Google Scholar
  6. 6.
    Mariotti D, Arcioni S (1983) Callus culture of Coronilla varia L. (crownvetch): Plant regeneration through somatic embryogenesis. Plant Cell Tissue Organ Culture 2:103–110Google Scholar
  7. 7.
    Matsuoka H, Kokichi H (1979) NAA-induced organogenesis and embryogenesis in hypocotyl callus of Solanum melongena L. J Exp Bot 30:363–370Google Scholar
  8. 8.
    Meredith CP (1979) Shoot development in established callus cultures of cultivated tomato (Lycopersicon esculentum Mill.). Z. Pflanzenphysiol 95:405–411Google Scholar
  9. 9.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  10. 10.
    Patterson BD, Paull R and Smillie RM (1978) Chilling resistance in Lycopersicon hirsutum Humb. & Bonpl., a wild tomato with a wide altitudinal distribution. Aust J Plant Physiol 5:609–617Google Scholar
  11. 11.
    Rick CM (1951) Hybrids between Lycopersicon esculentum Mill. and Solanum lycopersicoides Dun. Proc Nat Acad Sci 37:741–744Google Scholar
  12. 12.
    Santos AVP Dos, Outka DE, Cocking EC, Davey MR (1980) Organogenesis and somatic embryogenesis in tissues derived from leaf protoplasts and leaf explants of Medicago sativa. Z Pflanzenphysiol 99:261–270Google Scholar
  13. 13.
    Scott SJ, Jones RA (1982) Low temperature seed germination of Lycopersicon species evaluated by survival analysis. Euphytica 31:869–883Google Scholar
  14. 14.
    Sung ZR, Smith R, Horowitz J (1979) Quantitative studies of embryogenesis in normal and 5-methyltryptophan-resistant cell lines of wild carrot. The effects of growth regulators. Planta 147:236–240Google Scholar
  15. 15.
    Uchimiya H, Murashige T (1974) Evaluation of parameters in the isolation of viable protoplasts from cultured tobacco cells. Plant Physiol 54:936–944Google Scholar
  16. 16.
    Vallejos CE (1979) Genetic diversity of plants for response to low temperature and its potential use of crop plants. In: Lyons JM, Graham D, Raison JK (eds) Low Temperature Stress in Crop Plants, the Role of the Membrane. Academic Press, New York, pp. 473–489Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1985

Authors and Affiliations

  • L. W. Handley
    • 1
  • K. C. Sink
    • 1
  1. 1.Department of HorticultureMichigan State UniversityEast LansingUSA

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