Plant Cell, Tissue and Organ Culture

, Volume 5, Issue 3, pp 163–174 | Cite as

Production of betalains by suspension cultures of Chenopodium rubrum L.

  • Jochen Berlin
  • Sabine Sieg
  • Dieter Strack
  • Maria Bokern
  • Hans Harms


Red-violet cell suspension cultures of Chenopodium rubrum were found to accumulate the betacyanins amaranthin, celosianin and betanin and the betaxanthins vulgaxanthin I and vulgaxanthin II. Under a 16-h daylight regime the cells accumulated 0.3–0.4% betacyanins on a dry mass basis after 2–3 weeks of cultivation on the growth medium. Experiments to define a production medium for betacyanins failed with this habituated line. The accumulation could however be increased up to 1% or 100 mg betacyanins/1 by feeding tyrosine and by adaptation of the inoculum size to the nutrient concentration.

Key words

Chenopodium rubrum cell suspension cultures betalains accumulation media variation 


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Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • Jochen Berlin
    • 1
  • Sabine Sieg
    • 1
  • Dieter Strack
    • 2
  • Maria Bokern
    • 2
  • Hans Harms
    • 3
  1. 1.GBF-Gesellschaft f. Biotechnologische Forschung m.b.H.Braunschweig
  2. 2.Botanisches Institut der UniversitätKöln
  3. 3.Institut f. PflanzenernährungBundesforschungsastalt f. LandwirtschaftBraunschweigFRG

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