Plant Cell, Tissue and Organ Culture

, Volume 43, Issue 3, pp 207–213 | Cite as

Use of starch-gelled medium for tissue culture of some fruit crops

  • Richard H. Zimmerman
  • S. V. Bhardwaj
  • Ingrid M. Fordham
Original Research Paper


Six cultivars of apple and two of red raspberry consistently produced equal or significantly better shoot proliferation on modified Murashige and Skoog medium gelled with a mixture of corn starch and Gelrite than on the same medium gelled with agar. Two pear cultivars grown on starch-Gelrite medium produced hyperhydric shoots and almost no growth, but the addition of a polysaccharide hydric control (‘antivitrifying’) agent to the medium eliminated hyperhydricity. The resulting shoot proliferation equaled or exceeded that on the agar-gelled medium. The starch-Gelrite mixture is easy to prepare and gelling agent costs are only 10–15% of agar, or less if starch is purchased in bulk. Although the opaque gray-white medium makes it more difficult to detect internal contaminants, external contaminants are easily discerned.

Key words

antivitrification hyperhydricity polysaccharide 


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  1. Babbar SB & Gupta SC (1986) Effect of carbon source on Datura metel microspore embryogenesis and the growth of callus raised from microspore-derived embryos. Biochem. Physiol. Pflanz. 181: 331–338Google Scholar
  2. Batty N & Dunwell J (1989) Effect of maltose on the response of potato anthers in culture. Plant Cell Tiss. Organ. Cult. 18: 221–226Google Scholar
  3. Henderson WE & Kinnersley AM (1988) Corn starch as an alternative gelling agent for plant tissue culture. Plant Cell Tiss. Organ. Cult. 15: 17–22Google Scholar
  4. Kinnersley AM & Henderson WE (1988) Alternative carbohydrates promote differentiation of plant cells. Plant Cell Tiss. Organ. Cul. 15: 3–16Google Scholar
  5. Lydon J, Zimmerman RH, Fordham IM & Lusby WR (1993) Tissue culture and alkaloid production of Erythroxylum coca var. coca. J. Herbs Spices Medicinal Plants 2: 3–14Google Scholar
  6. Linsmaier EM & Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100–127Google Scholar
  7. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497Google Scholar
  8. Pasqualetto P-L, Zimmerman RH & Fordham I (1986) Gelling agent and growth regulator effects on shoot vitrification of ‘Gala’ apple in vitro. J. Amer. Soc. Hort. Sci. 111: 976–980Google Scholar
  9. SAS Institute, Inc. 1987 SAS/STAT guide for peronal computers, version 6 edition. SAS Institute, Inc., Cary, NCGoogle Scholar
  10. Sorvari S (1986a) The effect of starch gelatinized nutrient media in barley anther cultures. Ann. Agr. Fenn. 25: 127–133Google Scholar
  11. Sorvari S (1986b) Differentiation of potato tuber discs in barley starch gelatinized nutrient media. Ann. Agr. Fenn. 25: 135–138Google Scholar
  12. Sorvari S (1986c) Comparison of anther cultures of barley cultivars in barley-starch and agar gelatinized media. Ann. Agr. Fenn. 25: 249–254Google Scholar
  13. Strickland SG, Nichol JW, McCall CM & Stuart DA (1987) Effect of carbohydrate source on alfalfa somatic embryogenesis. Plant Sci. 48: 113–121Google Scholar
  14. Verma DC & Dougall DK (1977) Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures. Plant Physiol. 53: 81–85Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Richard H. Zimmerman
    • 1
  • S. V. Bhardwaj
    • 1
  • Ingrid M. Fordham
    • 1
  1. 1.Fruit LaboratoryU.S. Department of Agriculture, Agricultural Research ServiceBeltsvilleUSA
  2. 2.Department of BiotechnologyDr. Y.S. Parmar University of Horticulture and ForestrySOLANIndia

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