Plant Cell, Tissue and Organ Culture

, Volume 24, Issue 2, pp 73–77 | Cite as

Optimization of sucrose and inorganic nitrogen concentrations for somatic embryogenesis of birch (Betula pendula Roth.) callus cultures: A statistical approach

  • Anna Maria Nuutila
  • Ulrika Kurtén
  • Veli Kauppinen


The effects of different concentrations of sucrose and inorganic nitrogen on somatic embryogenesis of birch (Betula pendula Roth.) callus cultures were studied using Box-Wilson statistical experimental design. The experimental results were processed by regression analysis, and mathermatical models describing the effects of the selected variables were derived. On the basis of the modeling, maximum embryo production can be achieved with 35 mM total inorganic nitrogen and 20.8 g 1-1 sucrose in the medium.

Key words

Betula pendula Roth. birch callus culture mathematical models medium optimization somatic embryogenesis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ammirato PV (1983) Embryogenesis. In: Evans DA, Sharp WR, Ammirato PV & Yamada Y (Eds) Handbook of Plant Cell Culture, Vol 1 (pp 82–123). Macmillan Publishing Company, New YorkGoogle Scholar
  2. Box BEP & Wilson KB (1951) On the experimental attainment of optimum conditions. J. Roy. Statist Soc. 13: 1–45Google Scholar
  3. Cochran WG & Cox GM (1957) Experimental Designs, 2nd ed. John Wiley & Sons Inc., New YorkGoogle Scholar
  4. Gleddie S, Keller W & Setterfield G (1983) Somatic embryogenesis and plant regeneration from leaf explants and cell suspensions of Solanum melongena (eggplant). Can. J. Bot. 61: 656–666Google Scholar
  5. Horsch RB, King J & Jones GE (1980) Measurement of cultured plant cell growth on filter paper discs. Can. J. Bot. 58: 2402–2406Google Scholar
  6. Kurtén U, Nuutila AM, Kauppinen V & Rousi M (1990) Somatic embryogenesis in cell cultures of birch (Betula pendula Roth.). Plant Cell Tiss. Org. Cult. 23: 101–105Google Scholar
  7. Meijer WGM & Brown DCW (1987) Role of exogenous reduced nitrogen and sucrose in rapid high frequency somatic embryogenesis in Medicago sativa. Plant Cell Tiss. Org. Cult. 10: 11–19Google Scholar
  8. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497Google Scholar
  9. Simola LK (1985) Propagation of plantlets from leaf callus of Betula pendula F. pupurea. Scientia Hortic. 26: 77–85Google Scholar
  10. Tuominen U, Toivonen L, Kauppinen V, Markkanen P & Björk L (1989) Studies on growth and cardenolide production of Digitalis lanata tissue cultures. Biotech. Bioeng. 33: 558–562Google Scholar
  11. Törmälä T (1990) Genotype-fenotype interplay in micropropagation. In: Nijkamp HJJ, Van DerPlas LHW & VanAartrijk J (Eds) Progress in Plant Cellular and Molecular Biology (pp 102–107). Kluwer Academic Publishers, DordrechtGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Anna Maria Nuutila
    • 1
  • Ulrika Kurtén
    • 1
  • Veli Kauppinen
    • 1
  1. 1.Biotechnical laboratoryVTTEspooFinland

Personalised recommendations