, Volume 54, Issue 2, pp 201–204 | Cite as

Cryopreservation of shoot tips of Chrysanthemum morifolium and related species native to Japan

  • Seiichi Fukai
  • Masanori Goi
  • Michio Tanaka


The shoot tips of Chrysanthemum morifolium (syn. Dendranthema grandiflorum) and related species native to Japan were cryopreserved using preculture for 2 days, slow cooling (0.2°C/min) until −40°C with 10% dimethyl sulphoxide and 3% glucose prior to immersion into LN2 and rapid thawing. High survival rates were observed in 3 cultivars of chrysanthemum, 12 species and 2 interspecific hybrids, and slightly low survival rates in 3 species. The shoot regeneration rates of the frozen shoot tips varied from 9.4 to 100% depending on species. Shoot tips of chrysanthemum showed high viability even after a storage of 8 months in LN2. The thawed chrysanthemum shoot tips grew and flowered normally in a greenhouse under natural conditions.

Key words

Chrysanthemum cryopreservation shoot tips 





dimethyl sulphoxide


1-naphtalenacetic acid


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  1. Bajaj Y.P.S., 1895. Cryopreservation of germplasm of potato and cassava: viability of excised meristems cryopreserved up to four years. Indian J. Exp. Biol. 23: 285–287.Google Scholar
  2. Fukai S., 1989. Plant regeneration from shoot tips of Dianthus hybrida cryopreserved in liquid nitrogen up to 2 years. Plant Tissue Culture Letter. 6: 177–178.Google Scholar
  3. Fukai S., 1990. Cryopreservation of chrysanthemum shoot tips. Scientia Hort. 45: 167–174.CrossRefGoogle Scholar
  4. Fukai S. & M. Oe, 1990. Morphological observations of chrysanthemum shoot tips cultured after cryoprotection and freezing. J. Japan. Soc. Hort. Sci. 59: 383–387.Google Scholar
  5. Murashige T. & F. Skoog, 1962. A revised medium for rapid growth and bioassay with tobaco tissue cultures. Physiol. Plant. 15: 473–497.Google Scholar
  6. Power, J.B. & J.V. Chapman, 1985. Isolation, culture and genetic manipulation of plant protoplasts. p. 37–66. In: ‘Plant cell culture’ R.A. Dixon (Ed). IRA Press.Google Scholar
  7. Shibata, M., J. Kawata, M. Amano, T. Kameno, H. Yamagishi, T. Toyoda, T. Yamaguchi, M. Okimura & M. Uda, 1988. Breeding process and characteristics of ‘Moonlight’, an interspecific hybrid between Chrysanthemum morifolium Ramat. and C. pacificum Nakai (in Japanese with English summary). Bull. Natl. Res. Inst. Veg., Ornam. Plant & Tea Japan, Ser. A No. 2: 257–277.Google Scholar
  8. Toyoda T., 1987. New spray-type chrysanthemum: hybridmum (in Japanese) Garden life 26: 58.Google Scholar
  9. Towill L.E., 1984. Survival at ultra-low temperatures of shoot tips from Solanum tuberosum groups andigena, phureja, stenotomum, tuberosum, and other tuber-bearing Solanum species. Cryo-Letters. 5: 319–326.Google Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Seiichi Fukai
    • 1
  • Masanori Goi
    • 1
  • Michio Tanaka
    • 1
  1. 1.Lab. Floriculture, Fac. AgricultureKagawa UniversityKita-gunJapan

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