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Euphytica

, Volume 54, Issue 2, pp 201–204 | Cite as

Cryopreservation of shoot tips of Chrysanthemum morifolium and related species native to Japan

  • Seiichi Fukai
  • Masanori Goi
  • Michio Tanaka
Article
  • 99 Downloads

Summary

The shoot tips of Chrysanthemum morifolium (syn. Dendranthema grandiflorum) and related species native to Japan were cryopreserved using preculture for 2 days, slow cooling (0.2°C/min) until −40°C with 10% dimethyl sulphoxide and 3% glucose prior to immersion into LN2 and rapid thawing. High survival rates were observed in 3 cultivars of chrysanthemum, 12 species and 2 interspecific hybrids, and slightly low survival rates in 3 species. The shoot regeneration rates of the frozen shoot tips varied from 9.4 to 100% depending on species. Shoot tips of chrysanthemum showed high viability even after a storage of 8 months in LN2. The thawed chrysanthemum shoot tips grew and flowered normally in a greenhouse under natural conditions.

Key words

Chrysanthemum cryopreservation shoot tips 

Abbreviations

BA

6-benzylaminopurine

DMSO

dimethyl sulphoxide

NAA

1-naphtalenacetic acid

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Seiichi Fukai
    • 1
  • Masanori Goi
    • 1
  • Michio Tanaka
    • 1
  1. 1.Lab. Floriculture, Fac. AgricultureKagawa UniversityKita-gunJapan

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