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Plant Molecular Biology

, Volume 26, Issue 1, pp 473–479 | Cite as

A phenylalanine ammonia-lyase gene from melon fruit: cDNA cloning, sequence and expression in response to development and wounding

  • George Diallinas
  • Angelos K. Kanellis
Short Communications

Abstract

Phenylalanine ammonia-lyase (PAL) is the first enzyme of phenylpropanoid biosynthesis involved in the synthesis of a multiplicity of plant natural products. We have isolated and characterized a nearly fulllength cDNA clone (pmPAL-1) corresponding to a melon fruit (Cucumis melo L. var. reticulatus) gene coding for a protein which is highly similar to PAL from other lants. Melon fruit PAL is transcriptionally induced both in response to fruit ripening and wounding. PAL gene expression follows the kinetics of expression of the ethylene biosynthetic genes during fruit development. In contrast, ethylene biosynthetic genes show different induction kinetics compared to PAL expression in response to wounding. Similar results have been found for two other genes coding for enzymes involved in flavonoid biosynthesis (chalcone synthase, CHS; chalcone isomerase, CHI). Our results imply that regulation of defense gene expression in melon is a co-ordinated process in response to both ethylene and an ethylene-independent wound signal.

Key words

Cucumis melo melon phenylalanine ammonia-lyase gene expression ripening wounding 

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • George Diallinas
    • 1
  • Angelos K. Kanellis
    • 1
    • 2
  1. 1.Institute of Molecular Biology and Biotechnology (IMBB)Foundation for Research and Technology HellasCreteGreece
  2. 2.Institute of Viticulture and Vegetable CropsNational Agricultural Research FoundationCreteGreece

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