Plant Molecular Biology

, Volume 22, Issue 1, pp 171–176 | Cite as

Nucleotide sequence of a genomic gene encoding tritin, a ribosome-inactivating protein from Triticum aestivum

  • Noriyuki Habuka
  • Jiro Kataoka
  • Masashi Miyano
  • Hideaki Tsuge
  • Hideo Ago
  • Masana Noma
Update section Short communication


A genomic gene of tritin, a ribosome-inactivating protein (RIP) from Triticum aestivum, was cloned using a barley RIP gene as a probe. The 5′-non-coding region has potential TATA boxes and three sequences homologous to the binding sequence of the transcriptional activator protein Opaque-2 which activates maize RIP gene expression. The cloned DNA encoded tritin consits of 275 amino acids with no secretion signal sequence. The coding region of tritin was expressed in Escherichia coli using lac promoter and yielded a protein similar to the native one, as determined by SDS-polyacrulamide gel electrophoresis and immunological analysis.

Key words

expression genomic gene protein synthesis ribosome RNA N-glycosidase wheat germ 



Mirabilis antiviral protein


ribosome-inactivating protein


sodium dodecyl sulfate polyacrylamide gel electrophoresis


median of inhibitory concentration of L-[35S] methionine incorporation


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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Noriyuki Habuka
    • 1
  • Jiro Kataoka
    • 1
  • Masashi Miyano
    • 1
  • Hideaki Tsuge
    • 1
  • Hideo Ago
    • 1
  • Masana Noma
    • 1
  1. 1.Life Science Research LaboratoryJapan Tobacco, Inc.Yokohama, KanagawaJapan

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