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Plant Cell, Tissue and Organ Culture

, Volume 6, Issue 1, pp 95–104 | Cite as

In vitro clonal propagation of Pisum sativum L.

  • Miroslav Griga
  • Eva Tejklová
  • František J. Novák
  • Marie Kubaláková
Article

Abstract

A system of in vitro clonal propagation has been developed in Pisum sativum L. (cv. Bohatýr). A modified MS-medium supplemented with 20 μM 6-benzylaminopurine (BAP) and 0.1 μM α-naphthaleneacetic acid (NAA) was used to induce multiple shoot formation from shoot apices, axillary buds of the first normal leaf, axillary buds of the first and second primary scales and axillary buds of cotyledons of 4 to 6 day old pea seedlings. Meristem explants maintained a high proliferation ability in each subculture in the course of 20 months of the culture. Regenerated shoots were rooted in the same basal medium containing 5 μM NAA. Rooted plants were cultured in hydroponic pots filled with half-strength MS-medium to attain anthesis and seed maturity. The phenotypic uniformity of the regenerants was evaluated. Cytological investigation confirmed the diploid stage (2n=14) of regenerants and their progeny. Histological studies revealed that proliferating shoots originated from axillary and adventitious buds. In vitro propagation is discussed as related to pea breeding.

Key words

Pisum sativum L. pea shoot tip culture axillary bud culture regeneration organogenesis in vitro clonal propagation 

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Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • Miroslav Griga
    • 1
  • Eva Tejklová
    • 2
  • František J. Novák
    • 3
  • Marie Kubaláková
    • 3
  1. 1.OSEVA-Plant Breeding and Seed CorporationPlant Breeding Station for LegumesHorní MoštěniceCzechoslovakia
  2. 2.OSEVA-Plant Breeding and Seed CorporationResearch and Breeding Institute of Technical Crops and LegumesŠumperk-TemeniceCzechoslovakia
  3. 3.Institute of Experimental BotanyCzechoslovak Academy of SciencesOlomoucCzechoslovakia

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