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In vitro plantlet formation from adventitious buds on juvenile seedlings of Hinoki cypress (Chamaecyparis obtusa)


Adventitious buds were induced on one-week-old juvenile seedlings of Hinoki cypress on Campbell and Durzan's basic medium containing 10 µM (2.25 mg/l) benzyladenine (BA) and 0.027 µM (0.005 mg/l) naphthaleneacetic acid (NAA) and they elongated into shoots on the same medium lacking benzyladenine. About 70% of shoots could be rooted on the same basic medium containing 14.8 µM (3 mg/l) indole-3-butyric acid (IBA), 0.54 µM (0.1 mg/l) naphthaleneacetic acid and 2.66 µM (1 mg/l) riboflavin. These plantlets were potted in perlite. They were micropropagated about 100 times per seedling within 18 months.

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Present address (until 3rd, October 1986): Katsuaki Ishii, CSIRO Division of Forest Research, PO Box 4008, Canberra, A.C.T. 2600 Australia

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Ishii, K. In vitro plantlet formation from adventitious buds on juvenile seedlings of Hinoki cypress (Chamaecyparis obtusa). Plant Cell Tiss Organ Cult 7, 247–255 (1986).

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Key words

  • Hinoki cypress
  • Chamaecyparis obtusa
  • micropropagation
  • juvenile seedling
  • in vitro culture