Adventitious buds were induced on one-week-old juvenile seedlings of Hinoki cypress on Campbell and Durzan's basic medium containing 10 µM (2.25 mg/l) benzyladenine (BA) and 0.027 µM (0.005 mg/l) naphthaleneacetic acid (NAA) and they elongated into shoots on the same medium lacking benzyladenine. About 70% of shoots could be rooted on the same basic medium containing 14.8 µM (3 mg/l) indole-3-butyric acid (IBA), 0.54 µM (0.1 mg/l) naphthaleneacetic acid and 2.66 µM (1 mg/l) riboflavin. These plantlets were potted in perlite. They were micropropagated about 100 times per seedling within 18 months.
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Abo El-Nil MM, Milton W (1982) Method for asexual reproduction of coniferous trees. United States Patent No. 4353186
Campbell RA, Durzan DJ (1975) Induction of multiple buds and needles in tissue culture of Picea glauca. Can J Bot 53: 1652–1657
Cheng TY (1975) Adventitious bud formation in culture of Doutlas fir (Pseudotsuga menziesii (Mirb.) Franco). Plant Sci Lett 5:97–102
David A, David H (1977) Manifestations de diverses potentialités organogénés d'organes ou de fragments d;organes de Pin maritime (Pinus pinaster Sol.) en culture in vitro. C R Acad Sci Paris 248:Série D, 627–630
Durzan DJ (1982) Nitrogen metabolism and vegetative propagation of forest trees. In: Bonga JM, Durzan DJ (ed), Tissue Culture in Forestry. Martinus Nijhoff/Dr W. Junk Pub., pp 256–324
Furukoshi T (1985) Developmental studies in induction of strobili in a seed orchard of Chamaecyparis obtusa Sieb. et Zucc. For Tree Breed 134:28–31
Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 107:161–170
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204
Steinhart CE, Standifer LCJ, Skoog F (1961) Nutrient requirements for in vitro growth of spruce tissue. Am J Bot 48:465–472
Verma DC, Litvay JD, Johnson MA, Einspahr DW (1982) Media development for cell suspension of conifers. In: Fujiwara A (ed), Plant Tissue Culture 1982. Maruzen Co., Ltd., pp59–60
Wolter KE, Skoog F (1966) Nutrient requirements of Fraxinus callus cultures. Am J Bot 53:263–269
Present address (until 3rd, October 1986): Katsuaki Ishii, CSIRO Division of Forest Research, PO Box 4008, Canberra, A.C.T. 2600 Australia
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Ishii, K. In vitro plantlet formation from adventitious buds on juvenile seedlings of Hinoki cypress (Chamaecyparis obtusa). Plant Cell Tiss Organ Cult 7, 247–255 (1986). https://doi.org/10.1007/BF00037741
- Hinoki cypress
- Chamaecyparis obtusa
- juvenile seedling
- in vitro culture