An evaluation of antibiotics for the elimination ofXanthomonas campestris pv.pelargonii (Brown) fromPelargonium x domesticum cv. ‘Grand Slam’ explants in vitro
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A range of antibiotics was evaluated for activity againstXanthomonas campestris pv.pelargonii (Xep) on diagnostic sensitivity testing and plant tissue culture media. Many of the antibiotics showed reduced or no activity on the latter. Tetracycline and cefotaxime, chosen for further investigation, were screened for light stability under plant culture regimes. Tetracycline was inactivated in photosynthetic photon fluxes of 22 μmol m-2 s-1 and above. The minimum bacteriocidal concentration of cefotaxime was determined in bacteriological and plant tissue culture media. Cefotaxime was further tested for phytoxicity and ability to eliminate Xcp from deliberately infected explants. Cefotaxime was shown to eliminate contamination and stimulate the growth of the plant tissue cultures up 500 mg l-1.
Key wordsbacterial contamination cefotaxime tissue culture
diagnostic sensitivity testing medium
minimum bacteriocidal concentration
half-strength Murashige & Skoog (1962) basal plant tissue culture medium
Xanthomonas campestris pv.pelargonii
photosynthetic photon flux
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