Plant Cell, Tissue and Organ Culture

, Volume 39, Issue 1, pp 93–96 | Cite as

Micropropagation of safed musli (Chlorophytum borivilianum), a rare Indian medicinal herb

  • S. D. Purohit
  • Ashish Dave
  • Gotam Kukda
Research Note


In vitro clonal multiplication of safed musli (Chlorophytum borivilianum Sant. et. Fernand.), a rare Indian medicinal herb, has been achieved on Murashige and Skoog's (MS) medium supplemented with 22.2 μM benzyladenine using young shoot bases as explants. Shoots multiplied at a rate of four-fold every 3 weeks. All shoots rooted when transferred to MS medium with 3/4-strength inorganic and organic constituents and 9.8 μM indolebutyric acid and 67% of the micropropagated plants were successfully established in pots. Such plants produced normal fasciculated storage roots as in wild plants.

Key words

clonal multiplication tissue culture aphrodisiac herb 





2,4-dichlorophenoxyacetic acid


indoleacetic acid


indolebutyric acid


Murashige and Skoog (1962)


α-naphthaleneacetic acid


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arrillaga MCB & Segura J (1987) Somatic embryogenesis from hypocotyl callus cultures of Digitalis obscura L. Plant Cell Rep. 6: 223–226Google Scholar
  2. Balachandran SM, Bhat SR & Chandel KPS (1990) In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.). Plant Cell Rep. 8: 521–524Google Scholar
  3. Bhat SR, Kackar A & Chandel KPS (1992) Plant regeneration from callus cultures of Piper longum L. by organogenesis. Plant Cell Rep. 11: 525–528Google Scholar
  4. Bhojwani SS (1980) In vitro propagation of garlic by shoot proliferation. Scientia. Hort. 13: 47–52Google Scholar
  5. Jat RD & Bordia PC (1990) Propagation studies in safed musli (Chlorophytum spp.) In: Chaudhary BL, Aery NC & Katewa SS (Eds) Proceedings of National Symposium on Advances in Plant Sciences: Current Status & Emerging Challenges (pp 46) Department of Botany, Sukhadia University, UdaipurGoogle Scholar
  6. Kirtikar KR & Basu BD (1975) Liliaceae Chlorophytum. In: Kirtikar KR & Basu BD (Eds) Indian Medicinal Plants (pp 2508–2509) Bishen Singh Mahendra Pal Singh, Dehradun, IndiaGoogle Scholar
  7. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497Google Scholar
  8. Nayar MP & Sastry ARk (1988) Chlorophytum borivilianum. In: Nayar MP & Sastry ARk (Eds) Red Data Book of Indian Plants, Vol 2 (pp 142), Botanical Survey of India, Calcutta, IndiaGoogle Scholar
  9. Pandey R, Chandel KPS & Rao SR (1992) In vitro propagation of Allium tuberosum Rottl. ex. Spreng. by shoot proliferation. Plant Cell Rep. 11: 375–378Google Scholar
  10. Vasil IK (1991) Rationale for the scale-up and automation of plant propagation. In: Vasil IK (Ed) Scale-up and Automation in Plant Propagation (pp 1–5), Academic Press Inc., LondonGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • S. D. Purohit
    • 1
  • Ashish Dave
    • 1
  • Gotam Kukda
    • 1
  1. 1.Plant Biotechnology Laboratory, Department of BotanyM.L. Sukhadia UniversityUdaipurIndia

Personalised recommendations