Plant Cell, Tissue and Organ Culture

, Volume 10, Issue 3, pp 221–226

A liquid culture method for rescuing immature embryos of eastern cottonwood

  • Michael A. Savka
  • Jeffrey O. Dawson
  • Jalmer J. Jokela
  • Robert M. Skirvin
Short Communication


A one-step method to rescue immature embryos of eastern cottonwood (Populus deltoides Bartr.) is described. Plantlets developed from 83% of 25-day-old embryos grown in shaken culture on Murashige and Skoog (MS) liquid medium with 2.2 μm indole-3-acetic acid (IAA) and from 86% of embryos not supplemented with IAA. In contrast, when the MS medium was solidified with 0.8% agar, plantlets developed from 25% of 25-day-old embryos cultured on medium supplemented with IAA and from 28% of embryos in medium not supplemented with IAA. Eighty eight percent of all plantlets survived a gradual acclimitization to peat plugs in a greenhouse. The one-step liquid-culture method is an effective means of rescuing immature embryos by ovule culture from excised artificially-pollinated female branches in our cottonwood breeding program.

Key words

Embryo culture Embryo rescue Ovule culture Populus deltoides Bartr. 


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Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • Michael A. Savka
    • 1
  • Jeffrey O. Dawson
    • 1
  • Jalmer J. Jokela
    • 1
  • Robert M. Skirvin
    • 2
  1. 1.Department of ForestryUniversity of Illinois at Urbana-ChampaignUrbana-ChampaignUSA
  2. 2.Department of HorticultureUniversity of Illinois at Urbana-ChampaignUrbana-ChampaignUSA
  3. 3.Department of Plant PathologyUniversity of IllinoisUrbanaUSA

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