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Plant Cell, Tissue and Organ Culture

, Volume 35, Issue 3, pp 253–257 | Cite as

Agrobacterium rhizogens mediated transformation of Rauvolfia serpentina: Regeneration and alkaloid synthesis

  • B. D. Benjamin
  • G. Roja
  • M. R. Heble
Original Research Paper

Abstract

Shoot cultures of Rauvolfia serpentina infected with Agrobacterium rhizogenes 15834 produced tumourous tissue at the site of injection, which eventually developed callus with hairy roots. Sporadic shoot formation occurred from the hairy roots. The shoots were grown to maturity in the green house. The mature transformed plants (RST) showed distinct variations in their physiological characteristics. The flowers of the transformed plants were more delicate and less pigmented when compared to the flowers of the mature normal plants. The roots of the transformed plants were hairy with a number of lateral branches, whereas the roots of the normal plant had very few lateral branches. The biomass of the transformed plant was 86.39 g/plant (fresh wt), significantly higher than the normal plant which was 77.3 g/plant (fresh wt). The total alkaloid content in the mature transformed plant (0.073 g per plant) was similar to the normal plant (0.078 g per plant), although the hairy roots contained little alkaloid.

Key words

Agrobacterlum rhizogenes indole alkaloids Rauvolfia serpentina transgenic plants 

Abbreviations

MS

Murashige & Skoog's basal medium

MLS

modified Linsmaier & Skoog's basal medium

BA

benzyladenine

NAA

naphthalene acetic acid

TLC

thin layer chromatography

HPLC

high performance liquid chromatography

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • B. D. Benjamin
    • 1
  • G. Roja
    • 1
  • M. R. Heble
    • 1
  1. 1.Plant Biotechnology SectionBhabha Atomic Research CentreBombayIndia

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