Plant Molecular Biology

, Volume 17, Issue 4, pp 875–885 | Cite as

Sequence of three members and expression of a new major subfamily of glutelin genes from rice

  • Fumio Takaiwa
  • Kiyoharu Oono
  • David Wing
  • Akira Kato


Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the split between the two varieties. While corresponding exons of the subfamily B showed 80 to 88% nucleotide sequence homology, those exons were only 60–65% homologous to those of the glutelin A subfamily [15, 19, 24], distinguishing them from the subfamily A. Intron position and derived polypeptide structure, in addition to the nucleotide sequence, confirm the subfamily B members as glutelins. Analysis of RNA from seeds of different stages of development showed that the subfamily B members were expressed at the same time as those of subfamily A, demonstrating coordinated regulation of the two subfamilies.

Key words

glutelin multigene family Oriza sativa seed storage protein 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Baumlein, H, Wobus, U, Pustell, J, Kafatos, FC: The legumin gene family: Structure of a B type gene of Vicia faba and a possible legumin gene specific regulatory element. Nucl Acids Res 14: 2707–2720 (1986).Google Scholar
  2. 2.
    Breathnach, R, Chambon, P: Organization and expression of eukaryotic split genes coding for proteins. Annu Rev Biochem 50: 349–383 (1981).Google Scholar
  3. 3.
    Brown, JWS: A catalogue of splice junction and putative branch point sequences from plant introns. Nucl Acids Res 14: 9549–9559 (1986).Google Scholar
  4. 4.
    Domoney, C, Barker, D, Casey, R: The complete deduced amino acid sequences of legumin β-polypeptides from different genetic loci in Pisum. Plant Mol Biol 7: 467–474 (1986).Google Scholar
  5. 5.
    Feinberg, AP, Vogelstein, B: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).Google Scholar
  6. 6.
    Heidecker, G, Messing, J: Structural analysis of plant genes. Annu Rev Plant Physiol 37: 439–466 (1986).Google Scholar
  7. 7.
    Henikoff, S: Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351–359 (1984).Google Scholar
  8. 8.
    Kirihara, JA, Petri, JB, Messing, J: Isolation and sequence of a gene encoding a methionine-rich 10-kDa zein protein from maize. Gene 71: 359–370 (1988).Google Scholar
  9. 9.
    Leisy, DJ, Hnilo, J, Zhao, Y, Okita, TW: Expression of a rice glutelin promoter in transgenic tobacco. Plant Mol Biol 14: 41–50 (1990).Google Scholar
  10. 10.
    Lycett, GW, Croy, RD, Shirsat, AH, Boulter, D: The complete nucleotide sequence of a legumin gene from pea (Pisum sativum L.). Nucl Acids Res 12: 4493–4506 (1984).Google Scholar
  11. 11.
    Maniatis, T, Fritsch, EF, Sambrook, J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratories, Cold Spring Harbor, NY (1982).Google Scholar
  12. 12.
    Masumura, T, Kidzu, K, Sugiyama, Y, Mitsukawa, N, Hibino, T, Tanaka, K, Fujii, S: Nucleotide sequence of a cDNA encoding a major rice glutelin. Plant Mol Biol 12: 723–725 (1989).Google Scholar
  13. 13.
    Messing, J, Geraghty, D, Heidecker, G, Hu, NT, Kridl, J, Rugenstein, I: Plant Gene Structure. In Kosuge, T, Medith, CP, Hallaender, A (eds) Genetic Engineering of Plants, pp. 211–227. Plenum Press, New York (1983).Google Scholar
  14. 14.
    Nielsen, NC, Dickinson, CD, Cho, TJ, Thannh, VII, Scallon, BJ, Fisher, RL, Sims, TL, Drews, GN, Goldberg, RB: Characterization of the glycinin gene family in soybean. Plant Cell 1: 313–328 (1989).Google Scholar
  15. 15.
    Okita, TW, Hwang, YS, Hnilo, J, Kim, WT, Aryan, AP, Larson, R, Kirishnan, HB: Structure and expression of the rice glutelin multigene family. J Biol Chem 264: 12573–12581 (1989).Google Scholar
  16. 16.
    Pang, PP, Pruitt, RE, Meyerowitz, EM: Molecular cloning, genomic organization, expression and evolution of 12S seed storage protein genes of Arabidopsis thaliana. Plant Mol Biol 11: 805–820 (1988).Google Scholar
  17. 17.
    Sanger, F, Nicklen, S, Coulson, AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).Google Scholar
  18. 18.
    Takaiwa, F, Kikuchi, S, Oono, K: The structure of rice storage protein glutelin precursor deduced from cDNA. FEBS Lett 206: 33–35 (1986).Google Scholar
  19. 19.
    Takaiwa, F, Kikuchi, S, Oono, K: A rice glutelin family — A major type of glutelin mRNAs can be divided into two classes. Mol Gen Genet 208: 15–22 (1987).Google Scholar
  20. 20.
    Takaiwa, F, Ebinuma, H, Kikuchi, S, Oono, K: Nucleotide sequence of a rice glutelin gene. FEBS Lett 221: 43–47 (1987).Google Scholar
  21. 21.
    Takaiwa, F, Kikuchi, S, Oono, K: The complete nucleotide sequence of a new type cDNA coding for rice storage protein glutelin. Nucl Acids Res 17: 3289 (1989).Google Scholar
  22. 22.
    Takaiwa, F, Oono, K: Interaction of an immature seed specific trans-acting factor with the 5′ upstream region of rice glutelin gene. Mol Gen Genet 224: 289–293 (1990).Google Scholar
  23. 23.
    Takaiwa, F, Oono, K, Kato, A: Analysis of the 5′ flanking region responsible for the endosperm specific expression of a rice glutelin chimeric gene in transgenic tobacco. Plant Mol Biol 16: 49–58 (1991).Google Scholar
  24. 24.
    Takaiwa, F, Oono, K: Genomic DNA sequences of two new genes for new storage protein glutelin in rice. Japan J Genet 66: 161–171 (1991).Google Scholar
  25. 25.
    Takaiwa, F, Oono, K: Restriction fragment length polymorphism of glutelin genes from cultivated rice (Oriza saviva L.). Japan J Genet 66: 155–160 (1991).Google Scholar
  26. 26.
    Thomas, P: Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77: 5201–5205 (1980).Google Scholar
  27. 27.
    Vonder, Haar, RA, Allen, RD, Cohen, EA, Nessler, CL, Thomas, LT: Organization of the sunflower 11S storage protein gene family. Gene 74: 433–443 (1988).Google Scholar
  28. 28.
    Yamagata, H, Sugimoto, T, Tanaka, K, Kasai, Z: Biosynthesis of storage proteins in developing rice seeds. Plant Physiol 70: 1094–1100 (1982).Google Scholar
  29. 29.
    Zhao, WM, Gatehouse, JA, Boulter, D: The purification and partial amino acid sequence of a polypeptide from the glutelin fraction of rice grains: Homology to legumin. FEBS Lett 162: 96–102 (1983).Google Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Fumio Takaiwa
    • 1
  • Kiyoharu Oono
    • 1
  • David Wing
    • 2
  • Akira Kato
    • 2
  1. 1.Department of Cell BiologyNational Institute of Agrobiological ResourcesTsukuba, IbarakiJapan
  2. 2.Department of Molecular BiologyNational Institute of Agrobiological ResourcesTsukuba, IbarakiJapan

Personalised recommendations