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Euphytica

, Volume 22, Issue 3, pp 457–466 | Cite as

Double-bridge hybrids of Solanum bulbocastanum and cultivars of Solanum tuberosum

  • J. G. TH. Hermsen
  • M. S. Ramanna
Article

Summary

Solanum bulbocastanum (2n=2x=24) has valuable characters for potato breeding, but cannot be hybridized directly with S. tuberosum cultivars. Both S. acaule (2n=4x) and S. phureja (2n=2x) were used as bridging species. Triploid S. acaule × S. bulbocastanum were doubled with colchicine and the resulting fertile hexaploid F1's crossed with S. phureja. The triple hybrids obtained were tetraploid or nearly so. The two genomes of S. acaule in these triple hybrids probably pair preferentially, which may provoke pairing and possibly crossing over between the chromosomes of S. bulbocastanum and S. phureja.

More than 20000 pollinations of the triple hybrids with four potato cultivars had to be made to produce 40 quadruple hybrids. These highly vigorous hybrids varied greatly in many morphological characters, resistance to Phytophthora infestans, fertility and crossability. The chromosome numbers are 48 (24 hybrids), 49 and 46, but some higher ploidy levels (65, 66, 72 chromosomes) were found as well. Their origin is to be sought in the fusion of an unreduced egg cell from triple hybrids (either euploid or hypoploid) and a reduced male gamete from the cultivars. This view is corroborated by their extreme resistance to Phytophthora. Also some 48-chromosome hybrids are highly resistant, which may indicate introgression from S. bulbocastanum.

Most quadruple hybrids are readily inter-crossable and crossable as females with cultivars; several also as males. Two could be hybridized with S. bulbocastanum, but the few seeds dit not germinate.

Studies of pachytene stage of meiosis revealed the presence of a S. bulbocastanum chromosome in at least one tetraploid hybrid, which is highly resistant to Phytophthora. At metaphase I of meiosis chromosome associations higher than quadrivalents were not found. Except in one hybrid, the frequency of quadrivalents did not exceed one per cell and the average proportion of chromosomes associated as bivalents amounted to 90%.

The quadruple hybrids (‘double-bridge’ hybrids) appear good starting material for breeding programmes aimed at introducing genes from S. bulbocastanum into S. tuberosum cultivars.

Keywords

Colchicine Ploidy Level Phytophthora Potato Cultivar Phytophthora Infestans 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Hermsen J. G. Th., 1966. Crossability, fertility and cytogenetic studies in Solanum acaule × Solanum bulbocastanum. Euphytica 15: 149–155.Google Scholar
  2. Hermsen J. G. Th. & M. S.Ramanna, 1969. Meiosis in different F1-hybrids of Solanum acaule Bitt × S. bulbocastanum Dun and its bearing on genome relationship, fertility and breeding behaviour. Euphytica 18: 27–35.Google Scholar
  3. Hermsen J. G. Th. & A. J. E.deBoer, 1971. The effect of colchicine treatment on Solanum acaule and S. bulbocastanum; a complete analysis of ploidy chimeras in S. bulbocastanum. Euphytica 20: 170–179.Google Scholar
  4. Ramanna M. S. & J. G. Th.Hermsen, 1971. Somatic chromosome elimination and meiotic chromosome pairing in the triple hybrid 6x-(Solanum acaule × S. bulbocastanum) × 2x-S. phureja. Euphytica 20: 470–481.Google Scholar
  5. Toxopeus H. J., 1954. Leaf testing as a method of genetical analysis of immunity from Phytophthora infestans in potatoes. Euphytica 3: 233–240.Google Scholar

Copyright information

© H. Veenman en zonen b.v., Wageningen 1973

Authors and Affiliations

  • J. G. TH. Hermsen
    • 1
  • M. S. Ramanna
    • 1
  1. 1.Institute of Plant Breeding (IvP)Agricultural UniversityWageningenthe Netherlands

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