Plant Cell, Tissue and Organ Culture

, Volume 26, Issue 2, pp 83–88 | Cite as

Variation in polar-group content in lipids of cowpea (Vigna unguiculata) Cell cultures as a mechanism of haloadaptation

  • Rafael Vazquez-Duhalt
  • Lilia Alcaraz-Meléndez
  • Hubert Greppin
Article

Abstract

Callus and cell suspension cultures of cowpea (Vigna unguiculata) were induced with 2,4-dichlorophenoxyacetic acid and grown at different NaCl concentrations. The cell biomass yield and its total lipid content decreased with increasing salinity. However, while the hexose content in lipids was higher, the amount of lipid phosphorus was significantly lower in both agar and cell suspension cultures. Ion-transport rates with artificial membranes prepared with different lipid fractions showed that lipids from cells grown in a saline medium were less permeable to Na+ and to Cl- than those grown in a non-saline medium. Also the permeability of membranes prepared with glycolipids was lower than those prepared with phospholipids and whole lipids. Apparently, the increase of hexose/phosphorus ratio in membrane lipids is induced in response to the halo-adaptation process.

Key words

cowpea ion transport lipids membranes salinity Vigna unguiculata 

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Rafael Vazquez-Duhalt
    • 1
  • Lilia Alcaraz-Meléndez
    • 1
  • Hubert Greppin
    • 2
  1. 1.Centro de Investigaciones Biológicas de Baja California SurLa Paz Baja California SurMéxico
  2. 2.Département de Physiologie VégétaleUniversité de Genève, 3 place de l'UniversitéGenève 4Switzerland

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