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Plant Cell, Tissue and Organ Culture

, Volume 34, Issue 2, pp 149–155 | Cite as

Callus production, suspension culture and in vitro alkaloid yields of Ephedra

  • Niamh A. O'Dowd
  • Patrick G. McCauley
  • David H. S. Richardson
  • Graham Wilson
Original Research Papers

Abstract

The effects of a range of plant growth regulators on callus production in various Ephedra species were examined. Species examined were E. andina, E. distachya, E. equisitina, E. fragilis var, camplyopoda, E. gerardiana, E. intermedia, E. major ssp procera, E. minima and E. saxatilis. All species produced callus on modified MS medium supplemented with 0.25 μM kinetin and 5.0 μM 2,4-dichlorophenoxyacetic acid or 1-naphthaleneacetic acid. Neither indole-3-acetic acid nor 3-indolebutyric acid induced significant callus formation but the latter maintained growth of established callus cultures in several species. Suspension cultures of several species were established in MS medium supplemented with 0.25 μM kinetin and 5.0 μM 2,4-dichlorophenoxyacetic acid or 1-naphthaleneacetic acid. Sustained fresh weight doubling times of 70±7h were recorded for cell suspension cultures of E. andina grown in a semi-continous air-lift bubble bioreactor and a minimum doubling time of 56 h was recorded for E. andina in batch culture. It also proved possible to immobilise E. andina batch cultures in sodium alginate beads.

Neither parent plants or in vitro cultures of E. distachya, E. fragilis or E. saxatilis produced alkaloids. Trace quantities of 1-ephedrine and trace-0.14% dwt d-pseudoephedrine were produced by in vitro cultures of other species. The ability to produce alkaloid diminished to zero with successive subcultures.

Key words

alkaloids ephedrine in vitro culture pseudoephedrine Ephedra phytohormones 

Abbreviations

Eph

1-ephedrine

Peph

d-pseudoephedrine

RGR

relative growth rate

KIN

kinetin

2,4-d

2,4-dichlorophenoxyacetic acid

NAA

1-naphthaleneacetic acid

IBA

3-in-dolebutyric acid

IAA

indole-3-acetic acid

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Niamh A. O'Dowd
    • 1
  • Patrick G. McCauley
    • 1
  • David H. S. Richardson
    • 1
  • Graham Wilson
    • 2
  1. 1.School of Botany, Trinity CollegeUniversity of DublinDublinIreland
  2. 2.Department of BotanyUniversity College DublinDublinIreland

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