Plant Cell, Tissue and Organ Culture

, Volume 39, Issue 3, pp 269–272 | Cite as

Micropropagation of Thymus piperella

  • F. Sáez
  • P. Sánchez
  • A. Piqueras
Research Note


Explants from aseptically germinated seeds of Thymus piperella L. were induced to form shoots on modified Murashige and Skoog medium, the best yield being 5.1 shoots per explant when the medium contained 6.6 μM BA plus 2.8 μM IAA. Shoots could be rooted on the same basal medium supplemented with 2.8 μM IAA, and 71% of the plantlets were successfully acclimatized.

Key words

acclimatization plant growth regulator tissue culture 





modified MS culture medium


indoleacetic acid


Murashige & Skoog (1962) culture medium


α-naphthaleneacetic acid


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • F. Sáez
    • 1
  • P. Sánchez
    • 1
  • A. Piqueras
    • 2
  1. 1.Departamento de Biología Vegetal (Botánica), Facultad de BiologíaUniversidad de MurciaEspinardo (Murcia)Spain
  2. 2.Centro de Edafología y Biología Aplicada del Segura — C.S.I.C. (U.E.I. no. 3)MurciaSpain

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