Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 39, Issue 3, pp 269–272 | Cite as

Micropropagation of Thymus piperella

  • F. Sáez
  • P. Sánchez
  • A. Piqueras
Research Note

Abstract

Explants from aseptically germinated seeds of Thymus piperella L. were induced to form shoots on modified Murashige and Skoog medium, the best yield being 5.1 shoots per explant when the medium contained 6.6 μM BA plus 2.8 μM IAA. Shoots could be rooted on the same basal medium supplemented with 2.8 μM IAA, and 71% of the plantlets were successfully acclimatized.

Key words

acclimatization plant growth regulator tissue culture 

Abbreviations

BA

benzyladenine

CMS

modified MS culture medium

IAA

indoleacetic acid

MS

Murashige & Skoog (1962) culture medium

NAA

α-naphthaleneacetic acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adzet T & Passet J (1976) Estudio quimiotaxonómico de Thymus piperella L. Collectanea Bot. X: 5–11Google Scholar
  2. Alcaraz F, Sánchez-Gómez P & Correal E (1989) Catálogo de las plantas aromáticas, condimentarias y medicinales de la región de Murcia. Instituto Nacional de Investigaciones Agrarias, MadridGoogle Scholar
  3. Collet GF (1985) Enracinement amélioré lors de la production in vitro de rosiers. Rev. Suisse Viticult. Arboric. Hort. 17: 259–263Google Scholar
  4. Furmanowa M & Olszowska O (1980) Thymus vulgaris L. propagation through tissue culture. Acta Polon. Pharm. 37: 243–247Google Scholar
  5. Furmanowa M & Olszowska O (1992) Micropropagation of Thyme. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 19 (pp 230–243). Springer-Verlag, Berlin, HeidelbergGoogle Scholar
  6. Lê CL (1989) Microbouturage in vitro du thym. Rev. Suisse Viticult. Arboric. Hort. 21: 355–358Google Scholar
  7. Morales R (1986) Taxonomy of the genus Thymus (excluded the Section Serpyllum) and Thymbra at the Iberian Peninsula. Ph D Thesis. Ruizia 3. MadridGoogle Scholar
  8. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497Google Scholar
  9. Olszowska O (1982) Vegetative propagation of Thymus vulgaris L. in tissue culture and estimation of selected strains. Ph D Thesis. Med Acad, WarsawGoogle Scholar
  10. Olszowska O & Furmanowa M (1987) Micropropagation of thyme (Thymus vulgaris L.) from nodal segments. Herba Pol 33: 137–144Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • F. Sáez
    • 1
  • P. Sánchez
    • 1
  • A. Piqueras
    • 2
  1. 1.Departamento de Biología Vegetal (Botánica), Facultad de BiologíaUniversidad de MurciaEspinardo (Murcia)Spain
  2. 2.Centro de Edafología y Biología Aplicada del Segura — C.S.I.C. (U.E.I. no. 3)MurciaSpain

Personalised recommendations