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Plant Cell, Tissue and Organ Culture

, Volume 10, Issue 2, pp 91–99 | Cite as

The role of pH gradients across the plasmalemma of Catharanthus roseus and its involvement in the release of alkaloids

  • H. Bouyssou
  • A. Pareilleux
  • G. Marigo
Article

Abstract

During growth, Catharanthus roseus cells exhibit an acidification of the culture medium that may be controlled by Ca2+. With a view to enhance the productivity of alkaloids by plant cells, the effect of extracellular pH modifications on the excretion processes has been investigated. Ca2+ dependent proton pumping leads to the release of various lipophilic amine-like compounds (benzylamine, methylamine, nicotine) initially accumulated by the cells, but also facilitates the excretion of endogenous ajmalicine. Once released in the medium, these compounds are however taken up again by the cells, probably as the charged form. For the alkaloid contained in C. roseus some evidence suggests that the diffusible form comes from the cytosolic compartment and not from the storage vacuoles. This appears to be a major production limitation to the use of pH gradients in order to favour alkaloid excretion.

Key words

Lipophilic amines distribution Ajmalicine release pH gradients Catharanthus roseus 

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Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • H. Bouyssou
    • 1
  • A. Pareilleux
    • 2
  • G. Marigo
    • 1
  1. 1.Centre de Physiologie Végétale, UA CNRS no. 241Université Paul SabatierToulouse CédexFrance
  2. 2.Département du Génie Biochimique et Alimentaire, UA, CNRS no. 544Institut National des Sciences appliquéesToulouse CédexFrance

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