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Plant Cell, Tissue and Organ Culture

, Volume 19, Issue 2, pp 91–102 | Cite as

Non-invasive image analysis evaluation of growth during plant micropropagation

  • M. A. L. Smith
  • L. Art Spomer
  • M. J. Meyer
  • M. T. McClelland
Article

Abstract

Microcomputerized video image analysis was adapted for rapid, objective, and non-intrusive quantification of shoot growth and development for plants growing in vitro. Custom-developed staging arrangements were essential to insure accurate viewing and representation of the plants in each of three standard culture vessels. Shoot length measurements from digitized culture images were strongly correlated with length measured manually ex vitro. Image analysis weighted density measurements of proliferating microcultures (even with irregular growth habits) provided a reliable indicator of shoot culture fresh weight. Non-destructive time course evaluations of growth rate and quality were demonstrated.

Key words

image analysis manual measurement microcultures shoot culture 

Abbreviations

FW

fresh weight

WD

weighted density

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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • M. A. L. Smith
    • 1
  • L. Art Spomer
    • 1
  • M. J. Meyer
    • 1
  • M. T. McClelland
    • 1
  1. 1.Department of HorticultureUniversity of IllinoisUrbanaUSA

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