Plant Cell, Tissue and Organ Culture

, Volume 15, Issue 2, pp 113–124 | Cite as

Influence of virus and virus-like agents on the development of citrus buds cultured in vitro

  • V. Greño
  • L. Navarro
  • N. Duran-Vila
General Paper


Tissue culture in vitro was used to determine the effect of six major citrus virus and virus-like agents. Nodal stem segments from inoculated Pineapple sweet orange (Citrus sinensis (L.) Osb.), Mexican lime (C. aurantifolia (Christm.) Swing.) and Arizona Etrog citron 861-Sl (C. medica L.) were cultured in vitro to induce shoots. Some virus and virus-like agents had a marked effect on bud development and further recovery of plantlets. The number and size of the shoots that developed from each bud were affected as a result of infection. The effect depended on the specific virus, the isolate and the host-disease combination. The possible implications of these results are discussed.

Key words

bud tissue culture citrus virus citrus viroids Citrus sinensis Citrus aurantifolia Citrus medica 


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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • V. Greño
    • 1
  • L. Navarro
    • 1
  • N. Duran-Vila
    • 1
  1. 1.Instituto Valenciano de Investigaciones AgrariasApartado OficialValenciaSpain

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