Plant Cell, Tissue and Organ Culture

, Volume 15, Issue 2, pp 113–124 | Cite as

Influence of virus and virus-like agents on the development of citrus buds cultured in vitro

  • V. Greño
  • L. Navarro
  • N. Duran-Vila
General Paper

Abstract

Tissue culture in vitro was used to determine the effect of six major citrus virus and virus-like agents. Nodal stem segments from inoculated Pineapple sweet orange (Citrus sinensis (L.) Osb.), Mexican lime (C. aurantifolia (Christm.) Swing.) and Arizona Etrog citron 861-Sl (C. medica L.) were cultured in vitro to induce shoots. Some virus and virus-like agents had a marked effect on bud development and further recovery of plantlets. The number and size of the shoots that developed from each bud were affected as a result of infection. The effect depended on the specific virus, the isolate and the host-disease combination. The possible implications of these results are discussed.

Key words

bud tissue culture citrus virus citrus viroids Citrus sinensis Citrus aurantifolia Citrus medica 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Altman, A, Goren, R (1974) Growth and dormancy cycles in citrus bud cultures and their hormonal control. Physiol Plant 30: 240–245Google Scholar
  2. 2.
    Altman, A, Goren, R (1977) Horticultural and physiological aspects of citrus bud culture. Acta Hort 78: 51–60Google Scholar
  3. 3.
    Altman, A, Goren, R (1978) Development of citrus bud explants in culture. J Am Soc Hort Sci 103(1): 120–123Google Scholar
  4. 4.
    Bar-Joseph, M, Loebenstein, G, Cohen, J (1976) Comparison of particle characteristics and cytopathology of citrus tristeza virus with other morphologically similar viruses. In: Calavan, EC (Ed) Proc 7th Conf Inter Organ Citrus Virol, pp 39–45. University of California, RiversideGoogle Scholar
  5. 5.
    Bove, JM, Vogel, R (Ed) (1980) Description and illustration of virus and virus-like diseases of citrus. SETCO, IFAC, ParisGoogle Scholar
  6. 6.
    Childs JFL (Ed) (1968) Indexing procedures for 15 citrus diseases of citrus trees. Agric Handbook No 333. ARS, USDAGoogle Scholar
  7. 7.
    Duran-Vila N, Ortega V, Navarro L (in press) Morphogenesis and tissue cultures of three citrus species. Plant Cell Tiss Org CultGoogle Scholar
  8. 8.
    Duran-Vila N, Pina JA, Ballester JF, Juarez J, Roistacher CN, Rivera-Bustamante R, Semancik JS (in press) The citrus exocortis disease: a complex of viroid-RNAs. In: Proc 10th Conf Inter Organ Citrus VirolGoogle Scholar
  9. 9.
    Duran-Vila, N, Semancik, JS (1982) Effects of exogenous auxins on tomato tissue infected with the citrus exocortis viroid. Phytopathology 72: 777–781Google Scholar
  10. 10.
    Earle, ED (1978) Phytotoxin studies with plant cells and protoplasts. In: Thorpe, TA (Ed) Frontiers of plant tissue culture 1978, p. 363–372. The International Association for Plant Tissue Culture, Calgary, Alberta, CanadaGoogle Scholar
  11. 11.
    Favre, JM, Juncker, B (1987) In vitro growth of buds taken from seedlings and adult plant material in Quercus robur L. Plant Cell Tiss Org Cult 8: 49–60Google Scholar
  12. 12.
    Giladi, I, Altman, A, Goren, R (1977) Differential effects of sucrose, abscissic acid, and benzyladenine on shoot growth and callus formation in the abscission zone of excised citrus buds. Plant Physiol 59: 1161–1164Google Scholar
  13. 13.
    Gonsalves, D, Garnsey, SM (1976) Association of particle size with sedimentation velocity of the nucleoprotein components of citrus variegation and citrus leaf rugose viruses. In: Calavan, EC (Ed) Proc 7th Conf Inter Organ Citrus Virol, pp 109–115. University of California, RiversideGoogle Scholar
  14. 14.
    Horsch, RB, King, J (1983) A covert contaminant of cultured plant cells: elimination of a Hyphomicrobium sp. from cultures of Datura innoxia (Mill.). Plant Cell Tiss Org Cult 2: 21–28Google Scholar
  15. 15.
    Kitto, SL, Young, MJ (1981) In vitro propagation of Carrizo citrange. HortScience 16: 305–306Google Scholar
  16. 16.
    Marton, L, Duran-Vila, N, Lin, JJ, Semancik, JS (1982) Properties of cell cultures containing the citrus exocortis viroid. Virology 122: 229–238Google Scholar
  17. 17.
    Monette, PL (1986) Micropropagation of kiwifruit using non-axenic shoot tips. Plant Cell Tiss Org Cult 6: 73–82Google Scholar
  18. 18.
    Murashige, T (1977) Clonal crops through tissue culture. In: Barz, W, Reinhard, E, Zen, MH (Eds) Plant tissue culture and its bio-technological application, p 392–403. Springer-Verlag, BerlinGoogle Scholar
  19. 19.
    Murashige, T, Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497Google Scholar
  20. 20.
    Navarro L (1981) Effect of citrus exocortis viroid (CEV) on root and callus formation by stem tissue of Etrog citron (Citrus medica L.) cultured in vitro. Proc Int Soc Citriculture, Japan, pp 437–439Google Scholar
  21. 21.
    Navarro, L, Juárez, J, Ballester, JF, Pina, JA (1980) Elimination of some citrus pathogens producing psorosis-like leaf symptoms by shoot-tip grafting in vitro. In: Calavan, EC, Garnsey, SM, Timmer, LW (Eds) Proc 8th Conf Inter Organ Citrus Virol, pp 162–166. University of California, RiversideGoogle Scholar
  22. 22.
    Navarro, L, Roistacher, CN, Murashige, T (1976) Effect of size and source of shoot-tips on psorosis-A and exocortis content of navel orange plants obtained by shoot-tip grafting in vitro. In: Calavan, EC (Ed) Proc 7th Conf Inter Organ Citrus Virol, pp 194–197. University of California, RiversideGoogle Scholar
  23. 23.
    Roistacher, CN (1976) Detection of citrus viruses by graft transmission: a review. In: Calavan, EC (Ed) Proc 7th Conf Inter Organ Citrus Virol, pp 175–184 University of California, RiversideGoogle Scholar
  24. 24.
    Roistacher, CN, Nauer, EM, Wagner, RL (1980) Transmissibility of cachexia, dweet mottle, psorosis, tatterleaf and infectious variegation viruses on knife blades and its prevention. In: Calavan, EC, Garnsey, SM, Timmer, LW (Eds) Proc 8th Conf Inter Organ Citrus Virol, pp 225–229. University of California, RiversideGoogle Scholar
  25. 25.
    Semancik JS, Roistacher CN, Duran-Vila N (in press) Viroid RNA associated with the cachexia (xyloporosis) disease of citrus. In: Proc 10th Conf Inter Organ Citrus VirolGoogle Scholar
  26. 26.
    Shepherd, RI, Francki, RIB, Hirth, L, Hollings, M, Inonye, T, Macleod, R, Purcifull, DE, Sinha, RC, Tremaine, JH, Valenta, V, Wetter, C (1976) New groups of plant viruses approved by the international committee on taxonomy of viruses, September 1975. Intervirology 6: 181–184Google Scholar
  27. 27.
    Tisserat, B (1985) Embryogenesis, organogenesis and plant regeneration. In: Dixon, RA (Ed) Plant cell culture: a practical approach. Oxford: IRL Press, pp 79–105Google Scholar
  28. 28.
    Tran Thanh Van, KM (1981) Control of morphogenesis in in vitro cultures. Ann Rev Plant Physiol 32: 291–311Google Scholar
  29. 29.
    Wang, MC, Lin, JJ, Duran-Vila, N, Semancik, JS (1986) Alteration in cell wall composition and structure in viroid-infected cells. Physiol and Molecular Plant Path 28: 107–124Google Scholar
  30. 30.
    Wallace, JM (1957) Tristeza and seedling yellows of citrus. Plant Dis Rep 41: 394–397Google Scholar
  31. 31.
    Young, PM, Hutchins, AS, Canfield, ML (1984) Use of antibodies to control bacteria in shoot cultures of woody plants. Plant Sci Lett 34: 203–209Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • V. Greño
    • 1
  • L. Navarro
    • 1
  • N. Duran-Vila
    • 1
  1. 1.Instituto Valenciano de Investigaciones AgrariasApartado OficialValenciaSpain

Personalised recommendations