CO2 enrichment in vitro. Effect on autotrophic and heterotrophic cultures of Nicotiana tabacum (var. Samsun)
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Plantlets of Nicotiana tabacum (var. Samsun) were grown under CO2 enriched air supplied by a Warburg buffer. Growth of all plant parts was enhanced. The maximum growth increase was found for roots (120%).
Addition of 30 g.l-1 sucrose in the medium resulted in a three time faster growth. However, the effect of CO2 enrichment was still positive in these conditions, although less pronounced than in autotrophic cultures.
Key wordsautotrophy carbon dioxide enrichment heterotrophy in vitro Nicotiana tabacum
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