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Plant Molecular Biology

, Volume 18, Issue 2, pp 387–398 | Cite as

The glucosinolate-degrading enzyme myrosinase in Brassicaceae is encoded by a gene family

  • Jiaping Xue
  • Marit Lenman
  • Anders Falk
  • Lars Rask
Article

Abstract

A full-length cDNA clone (MB3) and three partial clones (MA1, MB1 and MB2) which encode myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1) were isolated from a Sinapis alba (white mustard) cDNA library. Nucleotide sequence analysis of these clones revealed that they are encoded by a gene family. Southern blot analysis with gene-specific probes showed that the gene family consists of a least two subfamilies (MA and MB) each with several members both in S. alba and in Brassica napus (oilseed rape). In Arabidopsis thaliana (wall cress) only three myrosinase genes seem to be present. Northern blot analysis indicated that all the myrosinase mRNA species have the same size, approximately 1.95 kb.

Key words

Arabidopsis thaliana Brassicaceae Brassica napus glucosinolate myrosinase Sinapis alba 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Jiaping Xue
    • 1
  • Marit Lenman
    • 1
  • Anders Falk
    • 1
  • Lars Rask
    • 1
  1. 1.Department of Cell ResearchSwedish University of Agricultural Sciences, Uppsala Genetic CenterUppsalaSweden

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