Plant Molecular Biology

, Volume 18, Issue 2, pp 315–325 | Cite as

Use of single-primer DNA amplifications in genetic studies of peanut (Arachis hypogaea L.)

  • Tracy Halward
  • Tom Stalker
  • Elizabeth LaRue
  • Gary Kochert


A recent approach to detecting genetic polymorphism involves the amplification of genomic DNA using single primers of arbitrary sequence. When separated electrophoretically in agarose gels, the amplification products give banding patterns that can be scored for genetic variation. The objective of this research was to apply these techniques to cultivated peanut (Arachis hypogaea L.) and related wild species to determine whether such an approach would be feasible for the construction of a genetic linkage map in peanut or for systematic studies of the genus. Two peanut cultivars, 25 unadapted germplasm lines of A. hypogaea, the wild allotetraploid progenitor of cultivated peanut (A. monticola), A. glabrata (a tetraploid species from section Rhizomatosae), and 29 diploid wild species of Arachis were evaluated for variability using primers of arbitrary sequence to amplify segments of genomic DNA. No variation in banding pattern was observed among the cultivars and germplasm lines of A. hypogaea, whereas the wild Arachis species were uniquely identified with most primers tested. Bands were scored (+/−) in the wild species and the PAUP computer program for phylogenetic analysis and the HyperRFLP program for genetic distance analysis were used to generate dendrograms showing genetic relationships among the diploid Arachis species evaluated. The two analyses produced nearly identical dendrograms of species relationships. In addition, approximately 100 F2 progeny from each of two interspecific crosses were evaluated for segregation of banding patterns. Although normal segregation was observed among the F2 progeny from both crosses, banding patterns were quite complex and undesirable for use in genetic mapping. The dominant behavior of the markers prevented the differentiation of heterozygotes from homozygotes with certainty, limiting the usefulness of arbitrary primer amplification products as markers in the construction of a genetic linkage map in peanut.

Key words

Arachis PCR arbitrary primers 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Arnold ML, Buckner CM, Robinson JT: Pollen-mediated introgression and hybrid speciation in Louisiana irises. Evolution (in press) (1991).Google Scholar
  2. 2.
    Burkart A: Estudios sistematicos sobre las Leguminosas-Hedisareas de La Republica Argentina y regiones adyacentes. De Darwiniana 3: 117–302 (1939).Google Scholar
  3. 3.
    Caetano-Anolles G, Bassam BJ, Gresshof PM: DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Bio/technology 9: 553–557 (1991).Google Scholar
  4. 4.
    Futuyma DJ: Evolutionary Biology. Sinauer Associates, Inc., Sunderland, MA (1986).Google Scholar
  5. 5.
    Gregory MP, Gregory WC: Exotic germplasm of Arachis L. interspecific hybrids. J Hered 70: 185–193 (1979).Google Scholar
  6. 6.
    Gregory WC, Gregory MP, Krapovickas A, Smith BW, Yarbrough JA: Structures and genetic resources of peanuts. In: Peanuts-Culture and Uses, pp. 47–133. American Peanut Research and Education Association, Stillwater, OK (1973).Google Scholar
  7. 7.
    Halward TM, Stalker HT, LaRue EA, Kochert GD: Relative levels of genetic variation detectable with molecular markers among unadapted germplasm resources of cultivated peanut and related wild species. Genome (in press) (1991).Google Scholar
  8. 8.
    Kochert GD, Halward TM, Branch WD, Simpson CE: RFLP variability in peanut (Arachis hypogaea L.) cultivars and wild species. Theor Appl Genet 81: 565–570 (1991).Google Scholar
  9. 9.
    Smartt J, Stalker HT: Speciation and cytogenetics in Arachis. In: Patee HE, Young CT (eds) Peanut Science and Technology, pp. 21–49. American Peanut Research and Education Society, Yoakum, TX (1982).Google Scholar
  10. 10.
    Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517 (1975).Google Scholar
  11. 11.
    Stalker HT: A morphological appraisal of wild species in section Arachis of peanuts. Peanut Sci 17: 117–122 (1990).Google Scholar
  12. 12.
    Stalker HT: A new species in section Arachis of peanuts with a D genome. Am J Bot 78: 630–637 (1991).Google Scholar
  13. 13.
    Stalker HT, Dhesi JS, Parry DC, Hahn JH: Cytological and infertility relationships of Arachis section Arachis. Am J Bot 78: 238–246 (1991).Google Scholar
  14. 14.
    Welsh J, McClelland M: Fingerprinting genomes using PCR with arbitrary primers. Nucl Acids Res 18: 7213–7218 (1990).Google Scholar
  15. 15.
    Welsh J, Peterson C, McClelland M: Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping. Nucl Acids Res 19: 303–306 (1991).Google Scholar
  16. 16.
    Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18: 6531–6535 (1990).Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Tracy Halward
    • 1
  • Tom Stalker
    • 2
  • Elizabeth LaRue
    • 1
  • Gary Kochert
    • 1
  1. 1.Department of BotanyUniversity of GeorgiaAthensUSA
  2. 2.Department of Crop ScienceNorth Carolina State UniversityRaleighUSA

Personalised recommendations