Photosynthesis Research

, Volume 32, Issue 3, pp 181–191 | Cite as

Mechanisms for controlling balance between light input and utilisation in the salt tolerant alga Dunaliella C9AA

  • Deborah Rees
  • Chin Bum Lee
  • D. James Gilmour
  • Peter Horton
Regular Paper
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Accepted:

Abstract

The yield of photosynthetic O2 evolution was measured in cultures of Dunaliella C9AA over a range of light intensities, and a range of low temperatures at constant light intensity. Changes in the rate of charge separation at Photosystem I (PS I) and Photosystem II (PS II) were estimated by the parameters ΦPS I and ΦPS II . ΦPS I is calculated on the basis of the proportion of centres in the correct redox state for charge separation to occur, as measured spectrophotometrically. ΦPS II is calculated using chlorophyll fluorescence to estimate the proportion of centres in the correct redox state, and also to estimate limitations in excitation delivery to reaction centres. With both increasing light intensity and decreasing temperature it was found that O2 evolution decreased more than predicted by either ΦPS I or ΦPS II. The results are interpreted as evidence of non-assimilatory electron flow; either linear whole chain, or cyclic around each photosystem.

Key words

chlorophyll fluorescence Dunaliella photosynthesis quantum efficiency 

Abbreviations

F0

dark level of chlorophyll fluorescence yield (PS II centres ‘open’)

Fm

maximum level of chlorophyll fluorescence yield (PS II centres ‘closed’)

Fv

variable fluorescence (Fm-F0)

PS I

Photosystem I

PS II

Photosystem II

P700

reaction centre chlorophyll(s) of PS I

qN

coefficient of non-photochemical quenching of chlorophyll fluorescence

qP

coefficient of photochemical quenching of fluorescence yield

qE

high-energy-state quenching coefficient

ΦPS I

yield of PS I

ΦPS II

yield of PS II

ΦS

yield of photosynthetic O2 evolution

ΦP

intrinsic yield of ‘open’ PS II centres

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Deborah Rees
    • 1
  • Chin Bum Lee
    • 1
  • D. James Gilmour
    • 1
  • Peter Horton
    • 1
  1. 1.Robert Hill Institute, Department of Molecular Biology and BiotechnologyUniversity of SheffieldWestern BankUK

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