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Plant Cell, Tissue and Organ Culture

, Volume 20, Issue 1, pp 41–46 | Cite as

In vitro clonal propagation of dioecious Carica papaya

  • O. Reuveni
  • D. R. Shlesinger
  • U. Lavi
Article

Abstract

A procedure for in vitro propagation of dioecious papaya clones is described. A high rate of success in culture estbalishment was obtained when axillary buds were taken from lateral shoots of hedged rooted cuttings grown in a greenhouse. Seasonal endophytic contamination was suppressed by shaking propagules for 24 h in 300 mgl-1 rifampicin or by incorporating it at 50 mgl-1 into the medium. Murashige & Skoog (MS) basal medium supplemented with 0.5 mgl-1 6-benzyladenine and 0.1 mgl-1 naphthaleneacetic acid was used for establishment and proliferation. The addition of 160 mgl-1 adenine sulfate improved multiplication and shoot growth. An elongation stage on MS medium supplemented with 1.0 mgl-1 kinetin and 0.05 mgl-1 naphthaleneacetic acid was necessary before rooting. Rooting was obtained at a high rate on half-strength macroelements of MS medium supplemented with 1.0 mgl-1 indole-3-butyric acid. Commercial plots of papaya plants obtained through this procedure already exist.

Key words

papaya endophytic contamination rifampicin micropropagation tissue culture 

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • O. Reuveni
    • 1
  • D. R. Shlesinger
    • 1
  • U. Lavi
    • 1
  1. 1.Institute of Horticulture, Agricultural Research OrganizationThe Volcani CenterBet DaganIsrael

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