Plant Cell, Tissue and Organ Culture

, Volume 38, Issue 1, pp 69–71 | Cite as

Multiplication of the endangered Indian pitcher plant (Nepenthes khasiana) through enhanced axillary branching in vitro

  • P. G. Latha
  • S. Seeni
Research Note


Axenic seedling-derived two- to three-node stem segments of Nepenthes khasiana Hook.f. were successfully cultured on Woody Plant Medium containing 2.2 μM benzyladenine to produce a 0.5–1.5 cm axillary shoot from each node in 7–8 weeks. The rapid growth along with the axillary branching of this shoot enabled amassing of 6–12 shoots during subculture. Excised shoots transferred to basal medium or rooted in medium containing 2.7 μM naphthaleneacetic acid produced typical pitchers at leaf tips. Rooted plants were established in pots at 90–95% survival rate.

Key words

ex situ conservation micropropagation 



ascorbic acid


activated charcoal


citric acid




indole-3-acetic acid


α-naphthaleneacetic acid


Knudson-C (1946) basal medium


Murashige & Skoog (1962) basal medium


Woody Plant basal medium (Lloyd & McCown 1980)


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • P. G. Latha
    • 1
  • S. Seeni
    • 1
  1. 1.Plant Biotechnology DivisionTropical Botanic Garden and Research InstitutePalodeIndia

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