Plant Cell, Tissue and Organ Culture

, Volume 12, Issue 3, pp 305–310 | Cite as

Clonal propagation of papaya in vitro

  • Wilna De Winnaar
Short Communications


A procedure for the rapid tissue culture propagation of papaya is being developed. Tissue culture methods using apices of nursery and orchard trees of Carica papaya cv. “Sunrise Solo” were evaluated. The explants were established in a modified Murashige and Tucker (1969) basal medium with half-strength inorganic salts, 0.5mgl-1 6-benzylaminopurine (BA) and 0.2mgl-1 naphthaleneacetic acid (NAA). Established explants were transferred to a proliferation medium consisting of Murashige and Tucker (1969) basal medium, 0.5mgl-1 BA and 0.1mgl-1 NAA, which caused extensive multiplication of shoots. Rooting was induced at a higher frequency by subculturing plantlets onto media with indole-3-butyric acid (IBA) than with NAA.

Key words

tissue culture propagation Carica papaya in vitro establishment proliferation rooting 


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  1. 1.
    Allan P (1964) Papaw grown from cuttings. Farming S Afr 39: 35–40Google Scholar
  2. 2.
    Litz RE & Conover RA (1977) Tissue culture propagation of papaya. Proc Fla State Hort Soc 90: 245–246Google Scholar
  3. 3.
    Litz RE & Conover RA (1978) In vitro propagation of papaya. Hort Science 13: 241–242Google Scholar
  4. 4.
    Litz RE & Conover RA (1981) Effect of sex type, season, and other factors on in vitro establishment and culture of Carica papaya L. explants. J Amer Soc Hort Science 106: 792–794Google Scholar
  5. 5.
    Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497Google Scholar
  6. 6.
    Murashige T & Tucker DPH (1969) Growth factor requirements of citrus tissue culture. Proc 1st Int Citrus Symp 3: 1155Google Scholar
  7. 7.
    Rajeevan MS & Pandey RM (1986) Lateral bud culture of papaya (Carica papaya L.) for clonal propagation. Plant Cell Tissue Organ Cult 6: 181–188Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Wilna De Winnaar
    • 1
  1. 1.Citrus and Subtropical Fruit Research InstituteNelspruitRepublic of South Africa

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