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Plant Cell, Tissue and Organ Culture

, Volume 12, Issue 3, pp 305–310 | Cite as

Clonal propagation of papaya in vitro

  • Wilna De Winnaar
Short Communications

Abstract

A procedure for the rapid tissue culture propagation of papaya is being developed. Tissue culture methods using apices of nursery and orchard trees of Carica papaya cv. “Sunrise Solo” were evaluated. The explants were established in a modified Murashige and Tucker (1969) basal medium with half-strength inorganic salts, 0.5mgl-1 6-benzylaminopurine (BA) and 0.2mgl-1 naphthaleneacetic acid (NAA). Established explants were transferred to a proliferation medium consisting of Murashige and Tucker (1969) basal medium, 0.5mgl-1 BA and 0.1mgl-1 NAA, which caused extensive multiplication of shoots. Rooting was induced at a higher frequency by subculturing plantlets onto media with indole-3-butyric acid (IBA) than with NAA.

Key words

tissue culture propagation Carica papaya in vitro establishment proliferation rooting 

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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Wilna De Winnaar
    • 1
  1. 1.Citrus and Subtropical Fruit Research InstituteNelspruitRepublic of South Africa

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