Plant Cell, Tissue and Organ Culture

, Volume 30, Issue 2, pp 135–140 | Cite as

Reconsideration of the term ‘vitrification’ as used in micropropagation

  • Debergh P. 
  • Aitken-Christie J. 
  • Cohen D. 
  • Grout B. 
  • Arnold S. von 
  • Zimmerman R. 
  • Ziv M. 


The term vitrification is currently used to describe two types of processes related to tissue-cultured plant material. The first is used to describe organs and tissues having an abnormal morphological appearance and physiological function. The second is used to describe the transition from liquid to solid state, i.e. the formation of ice during low temperature storage of in vitro cultured cells, tissues and organs. Use of the same term to define two greatly different processes in the same research area can only lead to confusion, especially for key words. Thus it is appropriate to reconsider the usage of vitrification in the first sense mentioned above. It is recommended that the term vitrification should no longer be used to indicate plant material with an abnormal morphological appearance and physiological function, and should be substituted by the term ‘hyperhydricity’.

Key words

hyperhydricity in vitro preferred usage vitrification 


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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Debergh P. 
    • 1
  • Aitken-Christie J. 
    • 2
  • Cohen D. 
    • 3
  • Grout B. 
    • 4
  • Arnold S. von 
    • 5
  • Zimmerman R. 
    • 6
  • Ziv M. 
    • 7
  1. 1.Laboratory of HorticultureState University GhentGhentBelgium
  2. 2.Forest Research InsituteRotoruaNew Zealand
  3. 3.Plant Physiology DivisionD.S.I.R.Palmerston NorthNew Zealand
  4. 4.The Merks EstateNOVALAL PLCGreat DunmowUK
  5. 5.Department Forest GeneticsSwedish University of Agricultural SciencesUppsalaSweden
  6. 6.USDA-ARS-Fruit LaboratoryBeltsvilleUSA
  7. 7.Department Agricultural BotanyThe Hebrew University of JerusalemRehovotIsrael

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