, Volume 16, Issue 1, pp 49–60 | Cite as

Colchicine induced polyploids in ryegrass

  • B. S. Ahloowalia


Studies on induction, isolation and stabilization of tetraploids in Lolium perenne L. (2n=14) are reported. These investigations revealed that:
  1. 1.

    The different varieties of ryegrass responded differentially to colchicine treatment.

  2. 2.

    Colchicine was mutagenic in addition to doubling chromosome number in ryegrass.

  3. 3.

    A high incidence of mixoploidy was obtained following colchicine treatment, which interfered with the fertility of the C0 tetraploids. The tillers, selected cytologically for tetraploid purity, had a higher seed set and 100-grain weight than either the mother plant or the diploid variety.

  4. 4.

    In the C1 generation, obtained from the interpollination of pure tetraploid tillers, about 92 per cent plants were tetraploid (2n=28), 7 per cent aneuploid (2n=25, 26,27) and less than one per cent diploid.

  5. 5.

    There was no significant difference in the percentage recovery of the tetraploids from small and large seeds in the C1 generation.



Colchicine Mother Plant Perennial Ryegrass Colchicine Treatment Tetraploid Plant 
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  1. 1.
    Ahloowalia, B. S., 1965. A root tip squash technique for screening chromosome number in Lolium. Euphytica 14: 170–172.CrossRefGoogle Scholar
  2. 2.
    Ahloowalia, B. S., 1965. Cytogenetic studies on natural hybrids between ryegrass and meadow fescue. Z. VererbLehre 97: 226–242.Google Scholar
  3. 3.
    Carnahan, H. L. and Hill, Helen, D., 1955. Lolium perenne L. × tetraploid Festuca elatior L. triploid hybrids and colchicine treatments for inducing autoallohexaploids. Agron. J. 47: 258–262.CrossRefGoogle Scholar
  4. 4.
    Cooper, J. P., 1963. Germination responses. Rep. Welsh Pl. Breed. Stn. 1962. 14–15.Google Scholar
  5. 5.
    Cooper, J. P. and Rhodes, I., 1964. DNA replication in climatic races. Rep. Welsh Pl. Breed. Stn. 1963. 18–19.Google Scholar
  6. 6.
    Dyck, P. L. and Rajhathy, T., 1965. A desynaptic mutant in Avena strigosa. Can. J. Genet. Cytol. 7: 418–421.Google Scholar
  7. 7.
    Franzke, C. J. and Ross, J. G., 1952. Colchicine-induced variants in Sorghum. J. Hered. 43: 107–115.Google Scholar
  8. 8.
    Franzke, C. J. and Sanders, Mary E., 1964. Classes of true-breeding diploid mutants obtained after colchicine treatment of Sorghum line Experimental 3. Bot. Gaz. 125: 170–178.CrossRefGoogle Scholar
  9. 9.
    Fürste, K., 1962. Welsches Weidelgras (Lolium multiflorum) Tetraploid. Z. PflZücht. 47: 369–387.Google Scholar
  10. 10.
    Griffiths, D. J. and Pegler, R. A. D., 1964. Natural crossing between diploid and tetraploid ryegrass varieties. Rep. Welsh Pl. Breed. Stn. 1963. 79–80.Google Scholar
  11. 11.
    Hertzsch, W., 1959. Beobachtungen an künstlich hergestellten polyploiden Futterflanzen. 3. Welsches Weidelgras (Lolium multiflorum). Z. PflZücht. 41: 271–293.Google Scholar
  12. 12.
    Hill, Helen, D. and Myers, W. M., 1944. Isolation of diploid and tetraploid clones from mixoploid plants of ryegrass (Lolium perenne L.), produced by treatment of germinating seeds with colchicine. J. Hered. 35: 359–361.Google Scholar
  13. 13.
    Hutton, E. M., 1957. Some effects of induced autopolyploidy in white clover, barrel medic and Wimmera ryegrass. J. Aust. Inst. Agric. Sci. 23: 227–231.Google Scholar
  14. 14.
    Myers, W. M., 1939. Colchicine induced tetraploidy in perennial ryegrass. J. Hered. 30: 499–504.Google Scholar
  15. 15.
    Myers, W. M., 1944. Cytological studies of a triploid perennial ryegrass and its progeny. J. Hered. 35: 17–23.Google Scholar
  16. 16.
    Roo,, 1952. Het verwekken van tetraploïdie in enkele voedergewassen door middel van colchicine. Meded. LandbHoogesch. OpzoekStns. Gent. 17 (2): 281–292.Google Scholar
  17. 17.
    Roo,, 1952. Een dubbel steriele variant in tetraploid materiaal van Lolium perenne. Meded. LandbHoogesch. OpzoekStns. Gent. 17 (3): 554–563.Google Scholar
  18. 18.
    Schumann, G., 1960. Eine neue Methode zur Colchicinierung von Gramineen und grosskörnigen Leguminosen. Züchter 30: 118–120.CrossRefGoogle Scholar
  19. 19.
    Shalygin, I. N., 1941. Production of tetraploids in Lolium by treating germinating seeds with colchicine. Comptes Rendus (Doklady) de l'Académie des Sciences de l'U.R.S.S. 30: 527–529.Google Scholar
  20. 20.
    Stuczynski, E., Kaminska, J. and Naleczynska, A., 1963. Tetraploid Westerwold ryegrass (Lolium multiflorum var. westerwoldichum). Genet. polon. 4: 303–308.Google Scholar
  21. 21.
    Sullivan, J. T., 1944. Further comparisons of plants with different chromosome numbers in respect to chemical composition. J. Amer. Soc. Agron. 36: 537–543.Google Scholar
  22. 22.
    Sullivan, J. T. and Myers, W. M., 1939. Chemical composition of diploid and tetraploid Lolium perenne L. J. Amer. Soc. Agron. 31: 869–871.Google Scholar
  23. 23.
    Tiemann, H., 1960. Die Erzeugung und Erkennung von Tetraploiden bei Gramineen. Züchter 30: 202–205.CrossRefGoogle Scholar
  24. 24.
    Wit, F., 1958. Tetraploid Italian ryegrass (Lolium multiflorum Lam.). Euphytica 7: 47–58.Google Scholar
  25. 25.
    Wit, F., 1959. Chromosome doubling and the improvement of grasses. Genet. Agraria 11: 97–115.Google Scholar
  26. 26.
    Wit, F. and Speckmann, G. J., 1955. Tetraploid Westerwolths ryegrass. Euphytica 4: 245–253.Google Scholar

Copyright information

© H. Veenman En Zonen N.V. 1967

Authors and Affiliations

  • B. S. Ahloowalia
    • 1
  1. 1.Plant Breeding DepartmentAgricultural InstituteOak Park, CarlowRepublic of Ireland

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