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Euphytica

, Volume 30, Issue 2, pp 239–246 | Cite as

Chromosome doubling through adventitious shoot formation on in vitro cultivated leaf explants from diploid interspecific potato hybrids

  • J. G. Th. Hermsen
  • M. S. Ramanna
  • S. Roest
  • G. S. Bokelmann
Article

Summary

Nearly 450 plantlets were produced from 51 diploid Solanum etuberosum x S. pinnatisectum F1 hybrids through adventitious shoot formation on in vitro cultivated rachis and petiole explants.

On the basis of phenotypical assessment of the ploidy level of 425 plants, 84.7% of the plants were scored as doubled or doubled twice. A cytological analysis of ploidy in the three layers L1.L2 and L3 of 112 plants revealed 83.9% complete doubling: periclinal ploidy chimeras were not found and only two sectorial ploidy chimeras were detected. Doubled plants were obtained from all 51 clones.

Various flower colours and epinastic leaves (in 1 clone) may be indications of mutagenesis through the treatment.

Index words

Solanum potato explant culture interspecific hybrid adventitious shoots in vitro chromosome doubling solid mutants 

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Copyright information

© Veenman B.V. 1981

Authors and Affiliations

  • J. G. Th. Hermsen
    • 1
  • M. S. Ramanna
    • 1
  • S. Roest
    • 2
  • G. S. Bokelmann
    • 2
  1. 1.Institute of Plant Breeding (IvP)Agricultural UniversityWageningenthe Netherlands
  2. 2.Foundation ITAL.Wageningenthe Netherlands

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