Plant Cell, Tissue and Organ Culture

, Volume 15, Issue 3, pp 223–231 | Cite as

Effects of auxins and cytokinins on epigenetic instability of callus-propagated Kalanchoe blossfeldiana Poelln.

  • A. Varga
  • L. H. Thoma
  • J. Bruinsma
General Paper


A main problem in the vegetative propagation of ornamental plants in vitro is the epigenetic instability of cells removed from their organized environment. With calluses of leaf explants of Kalanchoe blossfeldiana Poelln., cv. Yucatan, the role of plant growth regulators (PGRs) in the occurrence of fasciation was studied.

In various combinations of auxins and cytokinins, the auxin 2,4-D (2,4-dichlorophenoxyacetic acid) gave only deformed, inseparable shoot primordia. The most rapid callus induction with regeneration of well-developed sprouts was obtained with the natural IAA (indoleacetic acid) and Z (zeatin).

As a first symptom of fasciation, aberration in decussate phyllotaxis can be observed. At increasing concentrations of IAA + Z, this symptom gradually decreased but fasciation proper increased. The optimum concentration was at 1 μM for both PGRs. Reduction of exposure to the PGRs from six to three weeks reduced the epigenetic instability.

Key words

Kalanchoe in vitro propagation callus epigenetic instability fasciation plant growth regulators (PGRs) 


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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • A. Varga
    • 1
  • L. H. Thoma
    • 1
  • J. Bruinsma
    • 1
  1. 1.Department of Plant PhysiologyAgricultural UniversityWageningenThe Netherlands

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