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Plant Cell, Tissue and Organ Culture

, Volume 15, Issue 3, pp 211–222 | Cite as

Seasonal changes in explant viability and contamination of tissue cultures from mature Scots pine

  • Anja Hohtola
General Paper

Abstract

Explants from 10 to 40-year-old Scots pine trees (Pinus sylvestris L.) were cultured in vitro. Material was collected from Northern Finland once or twice a week during 1984–1987. excised shoot meristems and lower parts of the buds formed soft callus on modified MS medium. A seasonal effect was observed in the explant viability and degree of contamination. Callus proliferation was highest from explants collected in December and January and during the growing season from April to July, and lowest in February and during the autumn from September to November. It seemed that the bud metabolism at each particular time was rather persistent and affected the outcome of the experiments. Contamination was significantly higher from December to April. Organogenesis occurred only rarely.

Key words

Pinus sylvestris L. shoot tip culture callus proliferation browning 

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References

  1. 1.
    Abdullah AA, Yeoman MM, Grace J (1987) Micropropagation of mature Calabrian pine (Pinus brutia Ten.) from fascicular buds. Tree Physiol 3: 123–136Google Scholar
  2. 2.
    Arnold S von, Eriksson T (1981) In vitro studies of adventitious shoot formation in Pinus contorta. Can J Bot 59: 870–874Google Scholar
  3. 3.
    Ballenger LD, Huang FH (1984) Rooting of cuttings from mature Scotch pine trees. Arkansas Farm Res 33: 7Google Scholar
  4. 4.
    Bonga JM (1981) Organogenesis in vitro of tissues from mature conifers. In Vitro 17: 511–518Google Scholar
  5. 5.
    Bornman CH, Jansson E (1980) Organogenesis in cultured Pinus sylvestris tissue. Z Pflanzenphysiol 96: 1–6Google Scholar
  6. 6.
    Chalupa V, Durzan DJ (1973) Growth and development of resting buds of conifers in vitro. Can J For Res 3: 196–208.Google Scholar
  7. 7.
    Fridborg G, Pedersén M, Landström L-E, Eriksson T (1978) The effect of activated charcoal on tissue cultures: Adsorption of metabolites inhibiting morphogenesis. Physiol Plant 43: 104–106Google Scholar
  8. 8.
    Gupta PK, Durzan DJ (1985) Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Rep 4: 177–179Google Scholar
  9. 9.
    Grönroos R, Arnold S von (1985) Initiation and development of wound tissue and roots on hypocotyl cuttings of Pinus sylvestris in vitro. Physiol Plant 64: 393–401Google Scholar
  10. 10.
    Hohtola A, Kupila-Ahvenniemi S, Ohtonen R (1984) Seasonal changes in the cytoplasmic structures of sporogenous cells of Scotch pine. Ann Bot Fenn 21: 143–149Google Scholar
  11. 11.
    Horgan K, Aitken J (1981) Reliable plantlet formation from embryos and seedling shoot tips of radiata pine. Physiol Plant 53: 170–175Google Scholar
  12. 12.
    Häggman H, Hohtola A, Kupila-Ahvenniemi S (1985) Variation in the polysome assembly and incorporation of [3H]-uridine in the cells of pine buds during the cold season. Physiol Plant 65: 409–417Google Scholar
  13. 13.
    Johansen DA (1940) Plant microtechnique. McGraw-Hill Book Company, Inc, New York/London, pp 49–94Google Scholar
  14. 14.
    Kaul K (1986) Establishment of long-term callus cultures from mature white pine (Pinus strobus, Pinaceae). Amer J Bot 73: 242–245Google Scholar
  15. 15.
    Kopcewicz J, Michniewicz M, Kriesel K (1970) Auxins and growth inhibitors in pine (Pinus silvestris L.) and larch-trees (Larix decidua Mill.) of different age. Biologia 13: 139–146Google Scholar
  16. 16.
    Kupila-Ahvenniemi S (1977) Winter and spring changes in the microsporogenous cells of the Scotch pine as revealed by Feulgen photometry and Auramin fluorometry. Can J Bot 55: 1434–1442Google Scholar
  17. 17.
    Lindfors A, Kupila-Ahvenniemi S (1988) Metabolic and structural responses of pine buds under climatic chamber conditions. Physiol Plant 72: 407–413Google Scholar
  18. 18.
    Mudge KW (1986) Micropropagation of Mugo pine from embryonic and seedling explants. HortScience 21: 298–299Google Scholar
  19. 19.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497Google Scholar
  20. 20.
    Patel KR, Thorpe TA (1984) In vitro differentiation of plantlets from embryonic explants of lodgepole pine (Pinus contorta Dougl. ex Loud). Plant Cell Tiss Org Cult 3: 131–142Google Scholar
  21. 21.
    Rogozinska JH (1970) Culture of Scots pine callus and its nutritional requirements. Acta Soc Bot Pol 39: 151–160Google Scholar
  22. 22.
    Schenk RV, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199–204Google Scholar
  23. 23.
    Selby C, Harvey MR (1985) The influence of natural and in vitro bud flushing on adventitious bud production in Sitka spruce (Picea sitchensis (Bong.) Carr.) bud and needle cultures. New Phytol 100: 549–562Google Scholar
  24. 24.
    Skripachenko VV (1982) In vitro cultivation of tissues from seedlings of three species of pine. Sov Physiol 29: 162–167Google Scholar
  25. 25.
    Smith MAL, McCown B (1983) A comparison of source tissue for protoplast isolation from three woody plant species. Plant Sci Lett 28: 149–156Google Scholar
  26. 26.
    Sommer HE, Brown CL, Kormanik PP (1975) Differentiation of plantlets in long leafpine (Pinus palustris Mill.) tissue cultured in vitro. Bot Gaz 136: 196–200Google Scholar
  27. 27.
    Theodorsson-Norheim E (1986) Kruskal-Wallis test: BASIC computer program to perform nonparametric one-way analysis of variance and multiple comparisons on ranks of several independent samples. Comp Meth Progr Biomed 23: 57–62Google Scholar
  28. 28.
    Toribio M, Pardos JA (1981) In vitro organogenesis of Pinus sylvestris L. tissues. Afocel 14: 143–148Google Scholar
  29. 29.
    Tranvan H (1979) In vitro adventitious bud formation on isolated seedlings of Pinus silvestris L. Biol Plant 21: 230–233Google Scholar
  30. 30.
    Whitehill SJ, Schwabe WW (1975) Vegetative propagation of Pinus sylvestris. Physiol Plant 35: 66–71Google Scholar
  31. 31.
    Yli-Vakkuri P, Pelkonen P (1976) Rooting of Scots pine needle fascicles with different growth substances and media. Silva Fenn 10: 337–341Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Anja Hohtola
    • 1
    • 2
  1. 1.BiocenterUniversity of OuluOuluFinland
  2. 2.Department of BotanyUniversity of OuluOuluFinland

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