Plant Cell, Tissue and Organ Culture

, Volume 15, Issue 3, pp 211–222 | Cite as

Seasonal changes in explant viability and contamination of tissue cultures from mature Scots pine

  • Anja Hohtola
General Paper


Explants from 10 to 40-year-old Scots pine trees (Pinus sylvestris L.) were cultured in vitro. Material was collected from Northern Finland once or twice a week during 1984–1987. excised shoot meristems and lower parts of the buds formed soft callus on modified MS medium. A seasonal effect was observed in the explant viability and degree of contamination. Callus proliferation was highest from explants collected in December and January and during the growing season from April to July, and lowest in February and during the autumn from September to November. It seemed that the bud metabolism at each particular time was rather persistent and affected the outcome of the experiments. Contamination was significantly higher from December to April. Organogenesis occurred only rarely.

Key words

Pinus sylvestris L. shoot tip culture callus proliferation browning 


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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Anja Hohtola
    • 1
    • 2
  1. 1.BiocenterUniversity of OuluOuluFinland
  2. 2.Department of BotanyUniversity of OuluOuluFinland

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