Plant Cell, Tissue and Organ Culture

, Volume 22, Issue 3, pp 223–228 | Cite as

The relationship between genotype, tissue age and endogenous cytokinin levels on adventitious bud formation on leaves of Lachenalia

  • Josephina G. Niederwieser
  • J. van Staden
Article
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Abstract

The relationship between genotype, tissue age and endogenous cytokinin levels on adventitious bud formation on Lachenalia leaf tissue were investigated. The genotypes studied, showed a variation in bud formation. The hybrid explants responded differently to factorial combinations of BA and NAA. The growth regulators could not substitute for the regeneration potential of the genotype. Tissue age had a pronounced effect on regeneration potential. Young tissue formed the largest number of buds. An interaction between tissue age and genotype was detected. Cytokinin levels in young leaf tissue were higher than in older tissue. In young tissue no relationship was observed between the cytokinin level and the number of buds formed. However, in older tissue it appears as if a relatively low endogenous cytokinin level enhanced bud formation.

Key words

adventitious bud formation cytokinins effect of age and genotype Lachenalia 

Abbreviations

BA

benzyladenine

NAA

naphthalene-1-acetic acid

Z

zeatin

ZR

ribosylzeatin

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Josephina G. Niederwieser
    • 1
    • 2
  • J. van Staden
    • 1
  1. 1.UN/FRD Research Unit for Plant Growth and Development, Department of BotanyUniversity of NatalPietermaritzburgRSA
  2. 2.Vegetable and Ornamental Plant Research InstitutePretoriaRSA

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