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Plant Cell, Tissue and Organ Culture

, Volume 22, Issue 3, pp 205–211 | Cite as

Photosynthetic characteristics of Cymbidium plantlet in vitro

  • Toyoki Kozai
  • Hiroshi Oki
  • Kazuhiro Fujiwara
Article

Abstract

The photosynthetic characteristics of the Cymbidium plantlet in vitro cultured on Hyponex-agar medium with 2% sucrose were determined based on the measurements of CO2 concentration inside and outside of the culture vessels. The CO2 measurements were made with a gas chromatograph at a PPF (photosynthetic photon flux) of 35, 102 and 226 μmol m-2 s-1, a chamber air temperature of 15, 25 and 35°C and a CO2 concentration outside the vessel of approximately 350, 1100 and 3000 ppm. The net photosynthetic rates were determined on individual plantlets and were expressed on a dry weight basis. The steady-state CO2 concentration during the photoperiod was lower inside the vessel than outside the vessel at any PPF greater than 35 μmol m-2s-1 and at any chamber air temperature. The photosynthetic response curves relating the net photosynthetic rate, PPF, and CO2 concentration in the vessel and chamber air temperature were similar to those for Cymbidium plants grown outside and other C3 plants grown outside under shade. The results indicate that CO2 enrichment for the plantlets in vitro at a relatively high PPF would promote photosynthesis and hence the growth of chlorophyllous shoots/plantlets in vitro and that the plantlets in vitro would make photoautotrophic growth under environmental conditions favorable for photosynthesis.

Key words

CO2 enrichment Cymbidium in vitro photosynthesis 

Abbreviations

Cin

CO2 concentration in the culture vessel

Cout

CO2 concentration outside the vessel (in the culture room)

PPF

photosynthetic photon flux

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References

  1. 1.
    Capellades MQ (1989) Histological and ecophysiological study of the changes occurring during the acclimatization of in vitro cultures, Dissertation (pp 29–58), Gent University, Belgium.Google Scholar
  2. 2.
    Desjardins YA, Laforge F, Lussier C & Gosselin A (1988) Effect of CO2 enrichment and high photosynthetic photon flux on the development of autotrophy and growth of tissue-cultured strawberry, raspberry and asparagus plants. Acta Hortic. 230: 47–57.Google Scholar
  3. 3.
    Donnelly DJ & Vidaver WE (1984) Pigment content and gas exchange of red raspberry in vitro and ex vitro. J. Am. Soc. Hortic. Sci. 109: 177–181.Google Scholar
  4. 4.
    Fujiwara K, Kozai T & Watanabe I (1987) Measurements of carbon dioxide gas concentration in stoppered vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of the plantlets. J. Agric. Met. Japan 43: 21–30 (In Japanese with English summary and captions)Google Scholar
  5. 5.
    Fujiwara K, Kozai T & Watanabe I (1988) Development of a photoautotrophic tissue culture system for shoots and/or plantlets at rooting and acclimatization stages. Acta Hortic. 230: 153–158Google Scholar
  6. 6.
    George EF & Sherrington PD (1984) Plant Propagation by Tissue Culture. Exegetics Ltd., EnglandGoogle Scholar
  7. 7.
    Grout BWW & Ashton MJ (1978) Transplanting of cauliflower plants regenerated from meristem culture. II. Carbon dioxide fixation and the development of photosynthetic ability. Hortic. Res. 17: 65–71Google Scholar
  8. 8.
    Kako S, Mizuno N, Sugiyama A & Sakakibara K (1979) Studies on the development and flowering of Cymbidium. (14) Effects of temperature and light intensity on the development and photosynthesis. Extended Abstr. of Annual Autumn Meet. of Jap. Soc. for Hort. Sci. (pp 394–395)Google Scholar
  9. 9.
    Kozai T, Oki H & Fujiwara K (1987) Effects of CO2 enrichment and sucrose concentration under high photosynthetic photon fluxes on growth of tissue-cultured Cymbidium plantlets during the preparation stage, Symposium on plant micropropagation in horticultural industries, Arlon, Belgium (pp 135–141)Google Scholar
  10. 10.
    Kozai T, Kubota C & Watanabe I (1988) Effects of basal medium composition on the growth of carnation plantlets in auto- and mixotrophic tissue culture. Acta Hortic. 230: 159–166Google Scholar
  11. 11.
    Kozai T & Iwanami Y (1988) Effects of CO2 enrichment and sucrose concentration under high photon fluxes on plantlet growth of carnation (Dianthus caryophyllus L.) in tissue culture during the preparation stage. J. Jap. Soc. Hortic. Sci. 57: 279–288Google Scholar
  12. 12.
    Kozai T, Koyama Y & Watanabe I (1988) Multiplication of potato plantlets in vitro with sugar free medium under high photon photosynthetic flux. Acta Hortic. 230: 121–127Google Scholar
  13. 13.
    Kozai T (1990) Micropropagation under photoautotrophic conditions. In: Zimmerman R & Debergh PC (Eds) Micropropagation of Horticultural Crops. Kluwer Academic Publishers, Dordrecht. (in press)Google Scholar
  14. 14.
    Larcher W (1980) Physiological Plant Physiology (pp 94–102) Springer-VerlagGoogle Scholar
  15. 15.
    Pospisilova J, Catsky J, Solarova J & Ticha I (1987) Photosynthesis of plant regenerants, Specificity of in vitro conditions and plant response. Biol. Plant. (Praha) 29: 415–421Google Scholar
  16. 16.
    Pospisilova J, Solarova J, Catsky J & Ondrej M (1988) The photosynthetic characteristics during the micropropagation of tobacco and potato plants. Photosynthetica 22: 205–213Google Scholar
  17. 17.
    Shimada N, Tanaka F & Kozai T (1988) Effects of low O2 concentration on net photosynthesis of C3 plantlets in vitro. Acta Hortic. 230: 171–175Google Scholar
  18. 18.
    Solarova J (1989) Photosynthesis of plant regenerants. Diurnal variation in CO2 concentration in cultivation vessels resulting from plantlets photosynthetic activity. Photosynthetica 23: 100–107Google Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Toyoki Kozai
    • 1
  • Hiroshi Oki
    • 2
  • Kazuhiro Fujiwara
    • 1
  1. 1.Faculty of HorticultureChiba UniversityMatsudo, ChibaJapan
  2. 2.Chiba Pest Management CenterDaizenno, ChibaJapan

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