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Plant Cell, Tissue and Organ Culture

, Volume 22, Issue 3, pp 191–196 | Cite as

Selection for resistance to filtrates of Fusarium spp. in embryogenic cell suspension culture of Medicago sativa L.

  • Pavla Binarová
  • J. Nedělník
  • M. Fellner
  • Božena Nedbálková
Article

Abstract

A highly embryogenic cell suspension of alfalfa derived from a genotype sensitive to Fusarium oxysporum was successfully used for selection in vitro for resistance to culture filtrates of F. oxysporum, F. solani and F. avenaceum. Fifty two stable resistant cell lines were obtained and 500 plants regenerated from them. Among the 167 regenerants tested under glass there were 12–20% more plants with increased resistance to pathogens than in the group of plants regenerated from a control cell line. It was also found that the cell suspension cultures derived from genotypes of alfalfa with increased resistance to Fusarium spp. better tolerated filtrates of the pathogen. The results of a comparison of virulence of individual isolates of several species of Fusarium with toxicity of their filtrates to plants in vivo and in cell cultures were not unequivocal.

Key words

culture filtrate disease resistance embryogenic cell suspension culture Fusarium spp. Medicago sativa L. 

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Pavla Binarová
    • 1
  • J. Nedělník
    • 2
  • M. Fellner
    • 1
  • Božena Nedbálková
    • 2
  1. 1.Institute of Experimental BotanyCzechoslovak Academy of SciencesOlomoucCzechoslovakia
  2. 2.Research and Breeding Institute of Forage CropsTroubsko u BrnaCzechoslovakia

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