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Plant Cell, Tissue and Organ Culture

, Volume 29, Issue 2, pp 71–74 | Cite as

In vitro plant regeneration from leaf-derived callus in ginger (Zingiber officinale Rosc.)

  • K. Nirmal Babu
  • K. Samsudeen
  • M. J. Ratnambal
Article

Abstract

Excised tissues from young leaves of ginger cv. Maran were cultured on revised Murashige and Skoog medium supplemented with various concentrations of growth regulators. The presence of 2, 4-D in the culture medium at 9.0–22.6 μM resulted in callus growth. Organogenesis and plantlet formation occurred when the concentration of 2,4-D is reduced to 0.9 μM and with the addition of 44.4 μM BA into the medium. The rate of plant regeneration increased when the growth regulators are completely removed from the culture medium in the subsequent subcultures. The plantlets developed extensive root systems when they were put in MS liquid medium with 5.4 μM of NAA. The establishment of these plantlets in soil is about 80%.

Key words

leaf organogenesis 

Abbreviations

BA

N6-benzyladenine

NAA

α-naphthaleneacetic acid

2,4-D

2,4-dichlorophenoxyacetic acid

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References

  1. Bhagyalakshmi & Singh NS (1988) Meristem culture and micropropagation of a variety of ginger (Zingiber officinale Rosc.) with a high yield of oleoresin. J. Hort. Sci. 63: 321–327Google Scholar
  2. De Lange LH, Willers P & Nel M (1987) Elimination of nematodes from ginger (Zingiber officinale Rosc.) by tissue culture. J. Hort. Sci. 62: 249–252Google Scholar
  3. Hosoki T & Sagawa Y (1977) Clonal propagation of ginger (Zingiber officinale Rosc.) through tissue culture. HortScience 12: 45Google Scholar
  4. Hu CY & Wang PJ (1983) Meristem, shoot tip and bud cultures. In: Evans DA, Sharp WR, Ammirato PV & Yamada Y (Eds) Handbook of Plant Cell Culture, Vol I (pp 177–227). Macmillan Publishing Company, New YorkGoogle Scholar
  5. Ilahi I & Jabeen M (1987) Micropropagation of Zingiber officinale Rosc. Pakistan J. Bot. 19: 61Google Scholar
  6. Kulkarni DD, Khuspe SS & Mascarenhas AF (1984) Isolation of Pythium tolerant ginger by tissue culture. In: Potty SN (Ed) Proc. VI Symp Plantation Crops (pp 3–13). Rubber Research institute of India, Kottayam, Kerala.Google Scholar
  7. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497Google Scholar
  8. Noguchi Y & Yamakawa O (1988) Rapid clonal propagation of ginger (Z. officinale Rosc.) by roller culture. Japan J. Breeding 38: 437–442Google Scholar
  9. Pillai SK & Kumar NB (1982) Clonal multiplication of ginger in vitro. Indian J. Agric. Sci. 52: 397–399Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • K. Nirmal Babu
    • 1
  • K. Samsudeen
    • 1
  • M. J. Ratnambal
    • 1
  1. 1.National Research Centre for SpicesCalicut, KeralaIndia

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