Plant Cell, Tissue and Organ Culture

, Volume 24, Issue 3, pp 193–198 | Cite as

Somatic embryogenesis from immature embryos in meadowfoam (Limnanthes alba)

  • Darlene Southworth
  • Stephen Kwiatkowski
Article
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Accepted:

Abstract

Developing embryos from immature seeds were excised and cultured. Optimal proliferation of differentiated secondary embryos occurred on Murashige-Skoog media containing 7% sucrose, 0.1 μM 2,4-D, and 0.1–1.0 μM zeatin. Higher levels of auxins inhibited embryo proliferation. Secondary embryos were subcultured to produce more embryos. The results indicate the feasibility of clonal propagation of meadowfoam.

Key words

Limnanthes alba meadowfoam somatic embryogenesis tissue culture 

Abbreviations

2,4-D

2,4-dichlorophenoxyacetic acid

BA

6-Benzyladenine

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Darlene Southworth
    • 1
  • Stephen Kwiatkowski
    • 1
  1. 1.Department of BiologySouthern Oregon State CollegeAshlandUSA

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