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Plant Molecular Biology

, Volume 3, Issue 6, pp 333–336 | Cite as

Silent T-DNA genes in plant lines transformed by Agrobacterium tumefaciens are activated by grafting and by 5-azacytidine treatment

  • G. M. S. van Slogteren
  • P. J. J. Hooykaas
  • R. A. Schilperoort
Article

Summary

A shooty tumor induced by a shooter mutant of an octopine strain of Agrobacterium tumefaciens was cloned. One clone obtained (TS038) behaved aberrantly in that it grew as a shooty tumor tissue on phytohormone free medium, but did not contain octopine synthase activity. In line TS038 the genes for octopine synthase and for the enzymes involved in agropine and mannopine synthesis were present, but were not transcribed. However, the above genes became active in TS038 tumor shoots after grafting as well as after treatment with the hypomethylating agent 5-azacytidine. After an unusually long incubation period in the growth cabinet shoot cultures appeared to have developed small shoots from the top of the leaves. This unusual form of differentiation was found to be accompanied by the induction of octopine synthase activity.

Keywords

Agrobacterium tumefaciens crown gall plant tumor Ti plasmid T-DNA expression 5-azacytidine 

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Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1984

Authors and Affiliations

  • G. M. S. van Slogteren
    • 1
  • P. J. J. Hooykaas
    • 1
  • R. A. Schilperoort
    • 1
  1. 1.Dept. of Plant Molecular Biology, Molbas Group, Biochemical LaboratoryUniversity of LeidenLeidenThe Netherlands

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