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Photosynthesis Research

, Volume 25, Issue 3, pp 295–298 | Cite as

The chlorophyll fluorescence ratio F690/F730 in leaves of different chlorophyll content

  • Hartmut K. Lichtenthaler
  • Roman Hak
  • Ursula Rinderle
Regular Paper

Abstract

The red laser-induced chlorophyll-fluorescence induction kinetics of predarkened leaf samples were registered simultaneously in the 690 and 730 nm regions i.e., in the region of the two chlorophyll fluorescence emission maxima. From the induction kinetics the chlorophyll fluorescence ratio F690/F730 was calculated. The ratio F690/F730 shows to be dependent on the chlorophyll content of leaves. It is significantly higher in needles of damaged spruces (values of 0.45–0.9) than in normal green needles of healthy trees (values of 0.35–0.5). During development and greening of maple leaves the ratio F690/F730 decreases with increasing chlorophyll content. Determination of the ratio F690/F730 can be a suitable method of monitoring changes in chlorophyll content in a non-destructive way in the same leaves during development or the yellowish-green discolouration of needles of damaged spruces in the Black Forest with the typical tree decline symptoms.

Key words

Fluorescence induction kinetics maple leaves spruce needles two wavelength chlorophyll fluorometer 

Abbreviations

F690/F730

ratio of the fluorescence yield at the two fluorescence-emission maxima in the 690 and 730 nm regions

Fm

maximum fluorescence

Fs

steady-state fluorescence

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References

  1. French CS (1960) The chlorophylls in vivo and in vitro. In: Ruhland W (ed) Handbuch der Pflanzenphysiologie, Vol 5/1, pp 252–297Google Scholar
  2. Haitz M and Lichtenthaler HK (1988) The measurement of Rfd-values as plant vitality indices with the portable field fluorometer and the PAM-fluorometer. In: Lichtenthaler HK (ed) Applications of Chlorophyll Fluorescence, pp 249–254. Dordrecht, Kluwer Academic PublishersGoogle Scholar
  3. Kautsky H and Franck U (1943) Chlorophyllfluoreszenz und Kohlensäure-assimilation. Biochem Z 315: 139–232Google Scholar
  4. Krause GH and Weis E (1983) Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. Photosynth Res 5: 129–157Google Scholar
  5. Lichtenthaler HK (1987a) Chlorophyll fluorescence signatures of leaves during the autumnal chlorophyll breakdown. J Plant Physiol 131: 101–110Google Scholar
  6. Lichtenthaler HK (1987b) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148: 350–382Google Scholar
  7. Lichtenthaler HK and Rinderle U (1988) The role of chlorophyll fluorescence in the detection of stress conditions in plants. CRC Critical Reviews in Analytical Chemistry 19, Suppl I: 29–85Google Scholar
  8. Lichtenthaler HK, Rinderle U and Haitz M (1989) Seasonal Variations in Photosynthetic Activity of Spruces as determined by Chlorophyll Fluorescence. Ann Sci For 46 suppl: 483s-489sGoogle Scholar
  9. Nagel EM, Buschmann C and Lichtenthaler HK (1987) Photoacoustic spectra of needles as an indicator for the activity of the photosynthetic apparatus of healthy and damaged conifers. Physiol Plant 70: 427–437Google Scholar
  10. Papageorgiou G (1975) Chlorophyll fluorescence: An intrinsic probe of photosynthesis. In: Govindjee (ed) Bioenergetics of Photosynthesis, pp 319–371. New York, Academic PressGoogle Scholar
  11. Walker DA (1988) Some aspects of the relationship between chlorophyll a fluorescence and photosynthetic assimilation. In: Lichtenthaler HK (ed) Applications of Chlorophyll Fluorescence, pp 13–20. Dordrecht, Kluwer Academic PublishersGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Hartmut K. Lichtenthaler
    • 1
  • Roman Hak
    • 1
  • Ursula Rinderle
    • 1
  1. 1.Botanical Institute, Plant PhysiologyUniversity of KarlsruheKarlsruheFRG

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