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Photosynthesis Research

, Volume 25, Issue 3, pp 199–211 | Cite as

The effect of high-energy-state excitation quenching on maximum and dark level chlorophyll fluorescence yield

  • D. Rees
  • G. D. Noctor
  • P. Horton
Regular Paper

Abstract

The quenching of variable fluorescence yield (qN) and the quenching of dark level fluorescence yield (q0) directly atributable to high-energy-state fluorescence quenching (qE) was studied to distinguish between energy dissipation in the antenna and light harvesting complexes (antenna quenching) and energy dissipation at the reaction centres (reaction centre quenching). A consistent relationship was obtained between qN and q0 in barley leaves, the green alga Dunaliella C9AA and in pea thylakoids with 2,3,5,6-tetramethyl-p-phenylene diamine (DAD) as mediator of cyclic electron flow around PS 1. This correlated well with the relationship obtained using m-dinitrobenzene (DNB), a chemical model for antenna quenching, to quench fluorescence in Dunaliella C9AA or pea thylakoids. The results also correlated reasonably well with theoretical predictions by the Butler model for antenna quenching, but did not correlate with the predictions for reaction centre quenching. It is postulated that qE quenching therefore occures in the antenna and light harvesting complexes, and that the small deviation from the Butler prediction is due to PS 2 heterogeneity.

Keywords

Reaction Centre Energy Dissipation Chlorophyll Fluorescence Fluorescence Yield Dunaliella 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

9-aa

9-aminoacridine

DCMU

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea

EDTA

Ethylenediaminetetra-acetic acid

Hepes

4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid

Mes

2-(N-morpholino) prophanesulfonate

PS 1

photosystem 1

PS 2

photosystem 2

QA and QB

primary and secondary stable electron acceptors of photosystem 2

qN

non-photochemical fluorescence quenching coefficient

qE

high-energy-state fluorescence quenching coefficient

q0

quenching coefficient for F0

F0

dark level fluorescence yield

Fm

maximum fluorescence yield

Fv

variable fluorescence yield

Fv/Fm

ratio of variable to total fluorescence yield

DAD

2,3,5,6-tetramethyl-p-phenylene diamine

DNB

m-dinitrobenzene

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • D. Rees
    • 1
  • G. D. Noctor
    • 1
  • P. Horton
    • 1
  1. 1.Robert Hill Institute, Department of Molecular Biology and BiotechnologySheffield UniversitySheffieldUK

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