Photosynthesis Research

, Volume 7, Issue 1, pp 19–30 | Cite as

Effects of winter stress on photosynthetic electron transport and energy distribution between the two photosystems of pine as assayed by chlorophyll fluorescence kinetics

  • Gunnar öquist
  • Erling Ögren
FESPP Papers


The fluorescence kinetics of both intact needles and isolated chloroplasts of summer active and winter stressed Pinus sylvestris were measured at both room temperature and 77 K. It was confirmed that winter stress inhibited the photochemical capacity of photosystem II but also that winter stress caused the strongest inhibition of the electron transport at the site where the plastoquinone pool is reduced. Parallel analyses of the fluorescence characteristics of photosystem II (F693) and photosystem I (F729) during photosystem II trap closure furthermore revealed that the yield of spillover of excitation energy from photosystem II to photosystem I decreased upon winter stress. We suggest that this is because of an increased radiationless decay of excitation energy both at the reaction center and antennae levels of photosystem II. There is, however, also a possibility that the decreased yield of spill-over is accentuated by a partial detachment of the light harvesting chlorophyll a/b complex from photosystem II upon winter stress.

Key words

chlorophyll fluorescence energy transfer photoinhibition Pinus sylvestris winter stress 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15Google Scholar
  2. 2.
    Beddard GS and Porter G (1976) Concentration quenching in chlorophyll. Nature 260:366–367Google Scholar
  3. 3.
    Butler WL (1978) Energy distribution in the photochemical apparatus of photosynthesis. Ann Rev Plant Physiol 29:345–378Google Scholar
  4. 4.
    Kitajima M and Butler WL (1975) Excitation spectra for photosystem I and II in chloroplasts and the spectra characteristics of the distribution of quanta between the two photosystems. Biochim Biophys Acta 408:297–305Google Scholar
  5. 5.
    Kyle DJ, Ohad I and Arnzen CJ (1983) Selective thylakoid protein damage and repairing during photoinhibition. In: Sybesma C (ed) Advances in Photosynthesis Research, Vol 3, pp 67–70 Martin Nijhoff/Dr W Junk Publishers, The HagueGoogle Scholar
  6. 6.
    Lavorel J and Etienne A-L (1977) In vivo chlorophyll fluorescence. In: Barber J (ed) Primary Processes of Photosynthesis, pp. 203–268. Elsevier, Amsterdam-New York-OxfordGoogle Scholar
  7. 7.
    Linder S and Troeng E (1980) Photosynthesis and transpiration of 20-year-old Scots pine. In: Person T (ed) Structure and Function of Northern Coniferous Forests-An Ecosystem Study, Ecological Bulletins 32:165–181. Swedish Natural Science Research Council, StockholmGoogle Scholar
  8. 8.
    Martin B, Mårtensson O and Öquist G (1978) Effects of frost hardening and dehardening on photosynthetic electron transport and fluorescence properties in isolated chloroplasts of Pinus silvestris. Physiol Plant 43:297–305Google Scholar
  9. 9.
    Martin B, Mårtensson O and öquist G (1978) Seasonal effects on photosynthetic electron transport and fluorescence properties in isolated chloroplasts of Pinus silvestris. Physiol Plant 44:102–109Google Scholar
  10. 10.
    Martin B and Öquist G (1979) Seasonal and experimentally induced changes in the ultrastructure of chloroplast of Pinus silvestris. Physiol Plant 46:42–49Google Scholar
  11. 11.
    Ögren E and Öquist G (1984) Photoinhibition of photosynthesis in Lemna gibba as induced by the interaction between light and temperature. II. Photosynthetic electron transport. Physiol Plant 62:187–192Google Scholar
  12. 12.
    Ögren E and Öquist G (1984) Photoinhibition of photosynthesis in Lemna gibba as induced by interaction between light and temperature. III. Chlorophyll fluorescence at 77 K. Physiol Plant 62:193–200Google Scholar
  13. 13.
    Ögren E, Öquist G and Hällgren J-E (1984) Photoinhibition of photosynthesis in Lemna gibba as induced by the interaction between light and temperature. I. Photosynthesis in vivo. Physiol Plant 62:181–186Google Scholar
  14. 14.
    Öquist G (1982) Seasonally induced changes in acyl lipids and fatty acids of chloroplast thylakoids of Pinus silvestris. Plant Physiol 69: 869–875Google Scholar
  15. 15.
    Öquist G (1983) Effects of low temperature on photosynthesis. Plant Cell Envir 6:281–300Google Scholar
  16. 16.
    Öquist G and Martin B (1980) Inhibition of photosynthetic electron transport and formation of inactive chlorophyll in winter stressed Pinus silvestris. Physiol Plant 49:33–38Google Scholar
  17. 17.
    Öquist G, Mårtensson O, Martin B and Malmberg G (1978) Seasonal effects on chlorophyll-protein complexes isolated from Pinus silvestris. Physiol Plant 44: 187–192Google Scholar
  18. 18.
    Pisek A and Winkler E (1958) Assimilationsvermögen und Respirationen der Fichte (Picea excelsia Link.) in verschiedener Höhenlagen und der Zirbe (Pinus cembra L.) an der alpinen Waldgrenze. Planta 51: 518–543Google Scholar
  19. 19.
    Powles SB and Björkman O (1982) Photoinhibition of photosynthesis: effects on chlorophyll fluorescence at 77 K in intact leaves and in chloroplast membranes of Nerium oleander. Planta 156:97–107Google Scholar
  20. 20.
    Tranquillini W (1964) The physiology of plants at high altitudes. Ann Rev Plant Physiol 15:345–360Google Scholar
  21. 21.
    Zankel KL and Kok B (1972) Estimation of pool size and kinetic contents. In: Colowick SP and Kaplan NO (eds) Methods in Enzymology 24:218–228. Academic PressGoogle Scholar
  22. 22.
    Zelawski W and Kucharska J (1967) Winter depression of photosynthetic activity in seedling of Scots pine (Pinus silvestris L.). Photosynthetica 1:207–213Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1985

Authors and Affiliations

  • Gunnar öquist
    • 1
  • Erling Ögren
    • 1
  1. 1.Department of Plant PhysiologyUniversity of UmeåUmeåSweden

Personalised recommendations