Photosynthesis Research

, Volume 45, Issue 2, pp 111–119

The interactive effects of elevated CO2 and O3 concentration on photosynthesis in spring wheat

  • I. F. McKee
  • P. K. Farage
  • S. P. Long
Regular Paper

Abstract

This study investigated the interacting effects of carbon dioxide and ozone on photosynthetic physiology in the flag leaves of spring wheat (Triticum aestivum L. cv. Wembley), at three stages of development. Plants were exposed throughout their development to reciprocal combinations of two carbon dioxide and two ozone treatments: [CO2] at 350 or 700 μmol mol−1, [O3] at < 5 or 60 nmol mol−1. Gas exchange analysis, coupled spectrophotometric assay for RuBisCO activity, and SDS-PAGE, were used to examine the relative importance of pollutant effects on i) stomatal conductance, ii) quantum yield, and iii) RuBisCO activity, activation, and concentration. Independently, both elevated [CO2] and elevated [O3] caused a loss of RuBisCO protein and Vcmax. In combination, elevated [CO2] partially protected against the deleterious effects of ozone. It did this partly by reducing stomatal conductance, and thereby reducing the effective ozone dose. Elevated [O3] caused stomatal closure largely via its effect on photoassimilation.

Key words

carbon dioxide gas exchange ozone RuBisCO stomata Triticum aestivum L. 

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • I. F. McKee
    • 1
  • P. K. Farage
    • 1
  • S. P. Long
    • 1
  1. 1.Department of BiologyUniversity of EssexColchesterUK

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