Photosynthesis Research

, Volume 46, Issue 3, pp 467–472 | Cite as

Calibration of the Minolta SPAD-502 leaf chlorophyll meter

  • John Markwell
  • John C. Osterman
  • Jennifer L. Mitchell
Technical Communication


Use of leaf meters to provide an instantaneous assessment of leaf chlorophyll has become common, but calibration of meter output into direct units of leaf chlorophyll concentration has been difficult and an understanding of the relationship between these two parameters has remained elusive. We examined the correlation of soybean (Glycine max) and maize (Zea mays L.) leaf chlorophyll concentration, as measured by organic extraction and spectrophotometric analysis, with output (M) of the Minolta SPAD-502 leaf chlorophyll meter. The relationship is non-linear and can be described by the equation chlorophyll (μmol m−2)=10(M0.265), r2=0.94. Use of such an exponential equation is theoretically justified and forces a more appropriate fit to a limited data set than polynomial equations. The exact relationship will vary from meter to meter, but will be similar and can be readily determined by empirical methods. The ability to rapidly determine leaf chlorophyll concentrations by use of the calibration method reported herein should be useful in studies on photosynthesis and crop physiology.

Key words

chlorophyll leaf meter Minolta SPAD-502 meter 





SPAD-502 meter value


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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • John Markwell
    • 1
    • 2
  • John C. Osterman
    • 3
  • Jennifer L. Mitchell
    • 1
    • 2
  1. 1.Department of BiochemistryUniversity of NebraskaLincolnUSA
  2. 2.Department of AgronomyUniversity of NebraskaLincolnUSA
  3. 3.Department of School of Biological SciencesUniversity of NebraskaLincolnUSA

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