Advertisement

Photosynthesis Research

, Volume 3, Issue 3, pp 179–189 | Cite as

Electron transport and chloroplast ultrastructure of a chlorophyll deficient mutant of wheat

  • Freeman T. P. 
  • Duysen M. E. 
  • Olson N. H. 
  • Williams N. D. 
Article

Abstract

A non-lethal chlorophyll deficient mutation was induced by use of the chemical mutagen ethyl methanesulfonate. Chloroplasts from the control and mutant plants were found to be very similar ultrastructurally. Thylakoid membrane volume was only slightly greater in plastids from the control as compared with plastids from the mutant. The chlorophyll content of the mutant was reduced by over 60%. This decrease in chlorophyll was not accompanied by a similar decrease in electron transport. Uncoupled electron transport rate based on a unit chlorophyll basis was nearly twice as great for mutant chloroplasts as for control plastids. However, electron transport rate based on a unit membrane volume was similar in mutant and control plants. At high irradiance the relative quantum requirement of the control and mutant was similar when expressed on membrane volume.

Key words

chloroplast electron transport ultrastructure wheat mutant 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    ArmondPA, ArntzenCJ, BriantaisJM and VernotteC (1976) Differentiation of chloroplast lamellae. Light harvesting efficiency and grana development. Arch Biochem Biophys 175: 54–63Google Scholar
  2. 2.
    BoardmanNK and HighkinHR (1966) Studies on a barley mutant lacking chlorophyll b. I Photochemical activity of isolated chloroplasts. Biochem Biophys Acta 126: 189–199Google Scholar
  3. 3.
    DuysenME and FreemanTP (1974) The effects of moderate water deficit (stress) on wheat seedling growth and plastid pigment development. Physiol Plant 31: 262–266Google Scholar
  4. 4.
    DuysenME and FreemanTP (1980) Effects of ATP and successive additions of ADP on photosynthetic control ratios by isolated wheat chloroplasts. Photosynthetica 14: 73–78Google Scholar
  5. 5.
    HighkinHR, BoardmanNK and GoodchildDJ (1969) Photosynthetic studies of a pea-mutant deficient in chlorophyll. Plant Physiol 44: 1310–1320Google Scholar
  6. 6.
    HopkinsWG, HaydenDB and NeufferMG (1980) A light-sensitive mutant in maize (Zea mays L.). I Chlorophyll, chlorophyll-protein and ultrastructural studies. Z Pflazenphysiol 99: 417–426Google Scholar
  7. 7.
    HopkinsWG, GermanJB and HaydenDB (1980) A light-sensitive mutant in maize (Zea mays L.). II Photosynthetic properties. Z Pflazenphysiol 100: 15–24Google Scholar
  8. 8.
    KeckRW and DilleyRA (1970) Chloroplast composition and structure differences in a soybean mutant. Plant Physiol 46: 692–698Google Scholar
  9. 9.
    KeckRW, DilleyRA and KeB (1970) Photochemical characteristics in soybean mutant. Plant Physiol 46: 699–704Google Scholar
  10. 10.
    KeiichiroO, SchmidGH and StraubJ (1977) Genetic characteristics and high efficiency photosynthesis of an aurea mutant of tobacco. Plant Physiol 60: 150–156Google Scholar
  11. 11.
    KirkJTO and Tilney-BassettRA (1978) The Plastids. Amsterdam: Elsevier.Google Scholar
  12. 12.
    KoivuniemiPJ, TolbertNE and CarlsonPS (1981) Characterisation of the thylakoid membranes of the tobacco aurea mutant Su/su and of three revertant plants. Planta 151: 40–47Google Scholar
  13. 13.
    NielsenNC, SmillieRM, HenningenKW and vonWettsteinD (1979) Composition and function of thylakoid membranes from grana-rich and grana-deficient chloroplast mutants of barley. Plant Physiol 63: 174–182Google Scholar
  14. 14.
    ReevesSG, HallDA and BaltskeffskyH (1971) Photosynthetic control with endogenous and artificial electron acceptors. Biochem Biophys Res Comm 43: 459–466Google Scholar
  15. 15.
    RieskiJS, LumryR and SpikesJD (1959) The mechanism of the photochemical activity of isolated chloroplasts. III Dependence of velocity on light intensity. Plant Physiol 34: 293–300Google Scholar
  16. 16.
    SatohK, KatohS and TakamiyaA (1972) Light and dark rate-determined steps in electron transport reactions in spinach chloroplasts. Plant Cell Physiol 13: 885–897Google Scholar
  17. 17.
    SimpsonDJ and vonWettsteinD (1980) Macromolecular physiology of plastids XIV. Viridis mutants in barley: genetic, fluoroscopic and ultrastructural characterization. Carlsberg Res Commun 45: 283–314Google Scholar
  18. 18.
    WeibelER (1969) Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol 26: 235–302Google Scholar
  19. 19.
    WeibelER (1973) Stereological techniques for electron microcopic morphometry. In HayatMA ed. Principles and techniques of electron microscopy. Vol 3 New York: Van Nostrom ReinholdGoogle Scholar
  20. 20.
    WestKR and WiskichJT (1968) Photosynthetic control by isolated pea chloroplasts. Biochemical J 109: 527–532Google Scholar
  21. 21.
    Williams N, Joppa LR, Duysen ME and Freeman TP (1980) Inheritance of EMS-Induced chlorophyll- deficient mutants of common wheat, Triticum aestivum L. Agronomy Abstracts p 73Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1982

Authors and Affiliations

  • Freeman T. P. 
    • 1
  • Duysen M. E. 
    • 1
  • Olson N. H. 
    • 1
  • Williams N. D. 
    • 2
  1. 1.Botany DepartmentNorth Dakota State UniversityFargo
  2. 2.Research Geneticist, Agricultural Research ServiceUS Department of AgricultureFargo

Personalised recommendations