, Volume 114, Issue 3, pp 177–187 | Cite as

Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis

  • D. P. Sartory
  • J. U. Grobbelaar


The efficiency of pigment extraction forms the crux of the spectrophotometric analysis of chlorophyll a. The alcoholic solvents, methanol and ethanol, proved to be superior to acetone and acetone with DMSO. Homogenisation and sonication did not improve the extraction in the alcoholic solvents. Boiling at 100°C had an adverse effect whereas complete extraction of the pigments was obtained at the solvents boiling point and allowing the samples to stand for 24 h in the dark.


chlorophyll a extraction solvents homogenisation boiling sonication 


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Copyright information

© Dr W. Junk Publishers 1984

Authors and Affiliations

  • D. P. Sartory
    • 1
  • J. U. Grobbelaar
    • 2
  1. 1.Department of Environment AffairsHydrological Research InstitutePretoriaRepublic of South Africa
  2. 2.Institute for Environmental SciencesUniversity of the Orange Free StateBloemfonteinRepublic of South Africa

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