Stable transformation of lettuce cultivar South Bay from cotyledon explants
- 132 Downloads
Transgenic plants of lettuce cultivar (cv.) ‘South Bay’ were produced by using Agrobacterium tumefaciens vectors containing the β-glucuronidase (GUS) reporter gene and the NPT II gene for kanamycin resistance as a selectable marker. High frequency of transformation, based on kanamycin resistance and assays for GUS expression, was obtained with 24 to 72-h-old cotyledon explants cocultivated for 48 h with Agrobacterium tumefaciens. After the cocultivation period, the explants were placed in selection medium containing 50 or 100 mg l−1 of kanamycin, 100 mg l−1 cefotaxime and 500 mg l−1 carbenicillin for 10 days. Surviving explants were transferred every 14 days on shoot elongation medium. Progenies of R0 plants demonstrated linked monogenic segregation for kanamycin resistance and GUS activity.
Key wordsLactuca sativa regeneration tissue culture transgenic plant
Unable to display preview. Download preview PDF.
- Anonymous (1992) National Agricultural Statistics Service, USDA, WashingtonGoogle Scholar
- Bevan M & Goldsbrough A (1988) In: Hollaender A & Setiow J (Eds) Genetic Engineering Principles and Methods, Vol VI, Plenum Press, LondonGoogle Scholar
- Dusky JA, Stall WM & White JM (1988) Evaluation of herbicides for weed control in Florida lettuce production. Proc. Fla. State Hort. Sci. 101: 367–370Google Scholar
- Jefferson RA (1987) Assying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387–405Google Scholar
- Michelmore R, Marsh E, Seely S & Landry B (1987) Transformation of lettuce (Lactuca sativa) mediated by Agrobacterium tumefaciens. Plant Cell Rep. 6: 439–442Google Scholar
- Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497Google Scholar
- Owens LD & Smigocki AC (1988) Transformation of soybean cells using mixed strains of Agrobacterium tumefaciens and phenolic compounds. Plant Physiol. 88: 570–573Google Scholar
- Sheikholeslam SN & Weeks DP (1987) Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Mol. Biol. 8: 291–298Google Scholar