, Volume 70, Issue 1–2, pp 21–26 | Cite as

Characterization of five improved varieties and one wild accession of Lily of the Valley (Convallaria majalis L.) by isozyme analysis and assessment of genetic distances

  • P. Verron
  • M. Le Nard


Five cultivated forms and one wild accession of Lily of the Valley, Convallaria majalis L., have been investigated to estimate their genetic diversity and their genetic distances. Five enzyme systems were studied: esterase, acid phosphatase, peroxydase, phosphoglucomutase and superoxyde dismutase. Esterase system gave the lowest polymorphism, peroxydase and phosphoglucomutase systems gave the highest polymorphism. Grandiflora of Nantes, German and Fortunea Lily of the Valley appeared to be genetically different. In addition the Fortunea type which is a tetraploid form does not result in a polyploidization of any diploid types studied in this report. These cultivated forms could be the result of a natural and/or directed selection from wild Lily of the Valley.

Key words

Lily of the Valley Convallaria majalis isozymes genetic distances variation genetic diversity 





Acid phosphatase






Superoxyde dismutase


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anonymous, 1973. Le Muguet. Note technique C.N.I.H., Tec 02, 42 pp.Google Scholar
  2. Bradford, M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.Google Scholar
  3. Dalgren, R.M.T., H.T. Clifford & P.F. Yeo, 1985. The families of the monocotyledons. Springer Verlag, Berlin, pp. 137–140.Google Scholar
  4. Hebert, Y. & P. Vincourt, 1985. Mesure de la divergence génétique. In: M. Lefort-Buson & D. De Vienne (Eds.), Les Distances Génétiques, Estimations et Applications, pp. 23–27. INRA Pub.Google Scholar
  5. Le Nard, M. & P. Verron, 1993. Convallaria majalis L. In: A.A. De Hertog & M. Le Nard (Eds.) The Physiology of Flower Bulbs, pp. 249–256, Elsevier, Amsterdam.Google Scholar
  6. Reimann-Philipp, R., 1956. Die Erzeugung polyploïder Formen von Convallaria majalis sowie einige Beobachtungen über die Wirkung der Maiblumen Glykoside auf die Mitose. Zeitschrift für Pflanzenzüchtung 36: 289–304.Google Scholar
  7. Reimann-Philipp, R., 1986. 35 Jahre Züchtungsarbeit bei Maiblumen Erfahrungen und Ergebnisse. Deutscher Gartenbau 32: 1458–1459.Google Scholar
  8. Reimann-Philipp, R., 1987. Convallaria majalis (Lily of the Valley) improved as an ornamental plant after 35 years of breeding work. Acta Horticulturae 205: 65–69.Google Scholar
  9. Streveler, B., 1966. A taxonomic study of the genus Convallaria (Liliaceae). Thesis, University of Wisconsin, Madison, 89 pp.Google Scholar
  10. Utech, F.H. & S. Kawano, 1976. Floral vascular anatomy of Convallaria majalis L. and Convallaria keiskei Miq., (Liliaceae-Convallariinae). Bot. Mag. Tokyo 89: 173–182.Google Scholar
  11. Vallejos, C.E., 1983. Enzyme activity staining. In: S.D. Tanksley & T.J. Orton (Eds), Isozymes in Plant Genetics and Breeding, part A, pp. 469–516. Elsevier, Amsterdam.Google Scholar
  12. Vilmorin-Andrieux, S.A., 1939. Les fleurs de pleine terre. Librairie des Sciences et des Arts, Paris, pp. 703–704.Google Scholar
  13. Wetter, L. & J. Dyck, 1983. Isoenzyme analysis of cultured cells and somatic hybrids. In: D.A. Evans, W.R. Sharp, P.V. Ammirato & Y. Yamada (Eds.), Handbook of Plant Cell Culture, Vol. 1, Techniques for Propagation and Breeding, pp. 607–628. Mac Millan Pub., New York.Google Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • P. Verron
    • 1
  • M. Le Nard
    • 1
  1. 1.Station d'Amélioration de la Pomme de Terre et des Plantes à BulbesI.N.R.A.PloudanielFrance

Personalised recommendations